Presentation Title

Characterization and Identification of an Unknown Bacteria

Faculty Mentor

Hector Valenzuela

Start Date

18-11-2017 10:00 AM

End Date

18-11-2017 11:00 AM

Location

BSC-Ursa Minor 59

Session

Poster 1

Type of Presentation

Poster

Subject Area

biological_agricultural_sciences

Abstract

Listeria is a genus of rod-shaped, gram positive bacteria that are facultative anaerobic and non-endospore forming. It is commonly found in soil, water, animal products, and dairy. The purpose of these experiments was to develop experimental techniques that would identify an unknown bacteria through a series of different experiments including: gram staining, spore staining, acid-fast staining, determination of regular/irregular shape, catalase test, aerobic test, and PCA. A bacteria strain was provided and the experiment was done in a blind study format. The results of these experiment showed that the unknown bacteria is a gram positive rod with a regular shape, non-endospore-forming, non acid-fast, catalase positive, and facultative anaerobic. These characteristics that are observed during the testings lead to the conclusion that Listeria was the unknown bacteria. Confirmation was achieved through the amplification of species-specific gene, prfA, through PCR.

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Nov 18th, 10:00 AM Nov 18th, 11:00 AM

Characterization and Identification of an Unknown Bacteria

BSC-Ursa Minor 59

Listeria is a genus of rod-shaped, gram positive bacteria that are facultative anaerobic and non-endospore forming. It is commonly found in soil, water, animal products, and dairy. The purpose of these experiments was to develop experimental techniques that would identify an unknown bacteria through a series of different experiments including: gram staining, spore staining, acid-fast staining, determination of regular/irregular shape, catalase test, aerobic test, and PCA. A bacteria strain was provided and the experiment was done in a blind study format. The results of these experiment showed that the unknown bacteria is a gram positive rod with a regular shape, non-endospore-forming, non acid-fast, catalase positive, and facultative anaerobic. These characteristics that are observed during the testings lead to the conclusion that Listeria was the unknown bacteria. Confirmation was achieved through the amplification of species-specific gene, prfA, through PCR.