Presentation Title

Effect of antibiotics on natural transformation in Acenitobacter baumannii

Faculty Mentor

Maria Soledad Ramirez

Start Date

18-11-2017 10:00 AM

End Date

18-11-2017 11:00 AM

Location

BSC-Ursa Minor 38

Session

Poster 1

Type of Presentation

Poster

Subject Area

biological_agricultural_sciences

Abstract

Acinetobacter baumannii, a member of the highly resistant ESKAPE pathogens, has emerged over the last few decades. Horizontal genetic transfer (HGT) plays a key role in the evolution and adaptability of bacterial species as their capability to acquire and incorporate DNA into their genome. Little is known about how competence is induced in A. baumannii. We aimed to identify inducers of competence for natural transformation in this species.

Several kanamycin susceptible A. baumannii strains (A118, ATCC 17978, ATCC 19606 and A42) were challenged by sub-inhibitory concentrations of antibiotics (mitomycin C (MMC), nalidixic acid (NAL), meropenem (MEM)). Briefly, transformation assays using plasmid (pDSredAAC, conferring kanamycin resistance(KanR)) or genomic DNA (from strain Ab 144, known to carry a KanR determinant) were performed. Transformation events were scored by counting KanR colonies, while total CFUs were assessed by plating serial dilutions on LB agar.

MMC increased transformation frequencies in strain ATCC 17978 and in strain ATCC 19606 when transformed with plasmid and genomic DNA (P-value < 0.05), respectively. NAL increased (P-value < 0.05) transformation frequencies in strain A42 transformed with plasmid DNA while MEM increased (P-value < 0.05) transformation frequencies in A42, transformed with genomic DNA, and ATCC 17978, transformed with plasmid DNA (P-value < 0.05). MMC, NAL and MEM did not have a statistically significant effect on transformation frequencies with other strains, transformation frequencies did increase in all cases.

Our results show that antibiotics, that are present in hospital setting as well in the environment, can induce competence in A. baumannii.

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Nov 18th, 10:00 AM Nov 18th, 11:00 AM

Effect of antibiotics on natural transformation in Acenitobacter baumannii

BSC-Ursa Minor 38

Acinetobacter baumannii, a member of the highly resistant ESKAPE pathogens, has emerged over the last few decades. Horizontal genetic transfer (HGT) plays a key role in the evolution and adaptability of bacterial species as their capability to acquire and incorporate DNA into their genome. Little is known about how competence is induced in A. baumannii. We aimed to identify inducers of competence for natural transformation in this species.

Several kanamycin susceptible A. baumannii strains (A118, ATCC 17978, ATCC 19606 and A42) were challenged by sub-inhibitory concentrations of antibiotics (mitomycin C (MMC), nalidixic acid (NAL), meropenem (MEM)). Briefly, transformation assays using plasmid (pDSredAAC, conferring kanamycin resistance(KanR)) or genomic DNA (from strain Ab 144, known to carry a KanR determinant) were performed. Transformation events were scored by counting KanR colonies, while total CFUs were assessed by plating serial dilutions on LB agar.

MMC increased transformation frequencies in strain ATCC 17978 and in strain ATCC 19606 when transformed with plasmid and genomic DNA (P-value < 0.05), respectively. NAL increased (P-value < 0.05) transformation frequencies in strain A42 transformed with plasmid DNA while MEM increased (P-value < 0.05) transformation frequencies in A42, transformed with genomic DNA, and ATCC 17978, transformed with plasmid DNA (P-value < 0.05). MMC, NAL and MEM did not have a statistically significant effect on transformation frequencies with other strains, transformation frequencies did increase in all cases.

Our results show that antibiotics, that are present in hospital setting as well in the environment, can induce competence in A. baumannii.