Presentation Title

Effect of Caffeine on Biofilm Formation, Capsule Size, and Flagella on Pseudomonas aeruginosa

Faculty Mentor

John K. Chan

Start Date

18-11-2017 10:00 AM

End Date

18-11-2017 11:00 AM

Location

BSC-Ursa Minor 36

Session

Poster 1

Type of Presentation

Poster

Subject Area

biological_agricultural_sciences

Abstract

Caffeine is a stimulant that is consumed daily by millions of people in various beverages and pills. Pseudomonas aeruginosa (PA) is a gram-negative opportunistic microbe known to be the main cause for pneumonia in cystic fibrosis patients and is reported in many hospital-acquired infections. The number of flagella, length, and capsule formation may play a role in influencing the ability of PA to form biofilms. The objective of this study is to examine how caffeine affects the biofilm formation, capsule size, and flagella number and length. To determine the effect of caffeine on biofilm formation, different concentrations of caffeine ranging from 1x10­­-2 M to 1x10-12 M were added to a flat bottom 96-well microtiter plate containing a 1:5 dilution of the 24-hour culture of PA (ATCC 10145) in M63 liquid media. After 48 hours of incubation, biofilm was quantified by uptake of crystal violet and measured using a SpectraMax 190. To study capsule size and flagella, Maneval’s capsule stain and Ryu stain were used, respectively. ImageJ was used to measure the sizes of bacteria, capsule, and flagella. All data represent quadruplicate runs and were analyzed using a two sample T-test and measured at a significance of p<0.05. The current findings indicate that caffeine suppressed biofilm formation and bacteria size. There was an increase in the number of flagella and flagella length; however, caffeine did not affect capsule size and overall growth.

Summary of research results to be presented

Caffeine at higher concentrations significantly suppressed biofilm formation of Pseudomonas aeruginosa, but did not significantly decrease biofilm formation at lower concentrations, indicating that there is a dosage-dependent effect on Pseudomonas aeruginosa. Caffeine did not have a significant effect on capsule size of Pseudomonas aeruginosa, but caffeine did significantly decrease the bacteria size of Pseudomonas aeruginosa. Pseudomonas aeruginosa treated with caffeine showed an increase in flagella and flagellar length.

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Nov 18th, 10:00 AM Nov 18th, 11:00 AM

Effect of Caffeine on Biofilm Formation, Capsule Size, and Flagella on Pseudomonas aeruginosa

BSC-Ursa Minor 36

Caffeine is a stimulant that is consumed daily by millions of people in various beverages and pills. Pseudomonas aeruginosa (PA) is a gram-negative opportunistic microbe known to be the main cause for pneumonia in cystic fibrosis patients and is reported in many hospital-acquired infections. The number of flagella, length, and capsule formation may play a role in influencing the ability of PA to form biofilms. The objective of this study is to examine how caffeine affects the biofilm formation, capsule size, and flagella number and length. To determine the effect of caffeine on biofilm formation, different concentrations of caffeine ranging from 1x10­­-2 M to 1x10-12 M were added to a flat bottom 96-well microtiter plate containing a 1:5 dilution of the 24-hour culture of PA (ATCC 10145) in M63 liquid media. After 48 hours of incubation, biofilm was quantified by uptake of crystal violet and measured using a SpectraMax 190. To study capsule size and flagella, Maneval’s capsule stain and Ryu stain were used, respectively. ImageJ was used to measure the sizes of bacteria, capsule, and flagella. All data represent quadruplicate runs and were analyzed using a two sample T-test and measured at a significance of p<0.05. The current findings indicate that caffeine suppressed biofilm formation and bacteria size. There was an increase in the number of flagella and flagella length; however, caffeine did not affect capsule size and overall growth.