Presentation Title

Elucidating the Role of Novel Tctex Dynein Adaptor Proteins Through the Cell Cycle

Faculty Mentor

Dr. Jorge Z Torres

Start Date

18-11-2017 9:59 AM

End Date

18-11-2017 11:00 AM

Location

BSC-Ursa Minor 65

Session

Poster 1

Type of Presentation

Poster

Subject Area

biological_agricultural_sciences

Abstract

Abnormalities in the mechanisms of the cell cycle are the basis for various diseases, including cancer. Motor proteins, such as dyneins are one of the many driving forces of mitosis, transporting essential cargo across the cell. Recent studies on a family of Tctex dynein adaptor proteins, suggests that they have important roles in cell division. For instance, a novel protein identified as Tctex1d2, was found to be associated with mutated dynein intermediate chains that cause Short-rib polydactyly syndromes (SRPS). To further understand the role of these proteins in cell division and more broadly in human disease, this project seeks to characterize the localization and function of the Tctex proteins. The localization of Tctex1d1-4 proteins were observed by immunofluorescence and live cell microscopy. Additionally, Tctex protein levels were depleted by siRNA treatments and the resultant phenotypes will be characterized. Thus far characterization of Tctex1d2 shows an important role in cell division. Tctex1d2 has been found to localize to the cytokinetic bridge and spindle pole during mitosis. Knockdown of Tctex1d2 produced errors in cellular cleavage leading to binucleated cells. The results are indicative of a role for Tctex1d2 in cell division, potentially as a cargo substrate adaptor protein and we hypothesize that its associated cargo is essential for cell division. To test our hypothesis we plan on continuing to further characterize Tctex1d2 and the other Tctex proteins using cell and biochemical based assays. Proper characterization of these proteins will provide exciting opportunities to intervene in the regulatory pathways that arrest oncogenic cells.

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Nov 18th, 9:59 AM Nov 18th, 11:00 AM

Elucidating the Role of Novel Tctex Dynein Adaptor Proteins Through the Cell Cycle

BSC-Ursa Minor 65

Abnormalities in the mechanisms of the cell cycle are the basis for various diseases, including cancer. Motor proteins, such as dyneins are one of the many driving forces of mitosis, transporting essential cargo across the cell. Recent studies on a family of Tctex dynein adaptor proteins, suggests that they have important roles in cell division. For instance, a novel protein identified as Tctex1d2, was found to be associated with mutated dynein intermediate chains that cause Short-rib polydactyly syndromes (SRPS). To further understand the role of these proteins in cell division and more broadly in human disease, this project seeks to characterize the localization and function of the Tctex proteins. The localization of Tctex1d1-4 proteins were observed by immunofluorescence and live cell microscopy. Additionally, Tctex protein levels were depleted by siRNA treatments and the resultant phenotypes will be characterized. Thus far characterization of Tctex1d2 shows an important role in cell division. Tctex1d2 has been found to localize to the cytokinetic bridge and spindle pole during mitosis. Knockdown of Tctex1d2 produced errors in cellular cleavage leading to binucleated cells. The results are indicative of a role for Tctex1d2 in cell division, potentially as a cargo substrate adaptor protein and we hypothesize that its associated cargo is essential for cell division. To test our hypothesis we plan on continuing to further characterize Tctex1d2 and the other Tctex proteins using cell and biochemical based assays. Proper characterization of these proteins will provide exciting opportunities to intervene in the regulatory pathways that arrest oncogenic cells.