Presentation Title

Progress Towards Synthesis of Opioid Peptides to Addiction and Other Mood Disorders

Faculty Mentor

Michael J Ferracane

Start Date

18-11-2017 12:30 PM

End Date

18-11-2017 1:30 PM

Location

BSC-Ursa Minor 126

Session

Poster 2

Type of Presentation

Poster

Subject Area

physical_mathematical_sciences

Abstract

Addiction is a condition that affects over 21.4 million Americans. Antagonists of the kappa opioid receptor have shown promise to treat issues with addiction and other mood disorders. The macrocyclic tetrapeptide [D-Trp4]CJ-15,208 displays antagonist behavior in vitro but not in vivo. In the present study, we report progress towards the synthesis of an analog of [D-Trp4]CJ-15,208 that is intended to retain antagonist behavior in vivo. Solid-phase peptide synthesis (SPPS) was used to create the linear precursor to the desired analog; the identity and purity of this linear peptide was determined using mass spectrometry and high-performance liquid chromatography (HPLC). After the linear precursor was synthesized, the corresponding cyclic peptide was made in solution under dilute conditions. The crude cyclic peptide’s structure and purity was also confirmed by mass spectrometry and HPLC. It is to undergo purification via flash chromatography, and this work is currently ongoing.

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Nov 18th, 12:30 PM Nov 18th, 1:30 PM

Progress Towards Synthesis of Opioid Peptides to Addiction and Other Mood Disorders

BSC-Ursa Minor 126

Addiction is a condition that affects over 21.4 million Americans. Antagonists of the kappa opioid receptor have shown promise to treat issues with addiction and other mood disorders. The macrocyclic tetrapeptide [D-Trp4]CJ-15,208 displays antagonist behavior in vitro but not in vivo. In the present study, we report progress towards the synthesis of an analog of [D-Trp4]CJ-15,208 that is intended to retain antagonist behavior in vivo. Solid-phase peptide synthesis (SPPS) was used to create the linear precursor to the desired analog; the identity and purity of this linear peptide was determined using mass spectrometry and high-performance liquid chromatography (HPLC). After the linear precursor was synthesized, the corresponding cyclic peptide was made in solution under dilute conditions. The crude cyclic peptide’s structure and purity was also confirmed by mass spectrometry and HPLC. It is to undergo purification via flash chromatography, and this work is currently ongoing.