Presentation Title

Proliferative Effects of Rutin and Chlorogenic Acid on HUVECs

Faculty Mentor

P. Matthew Joyner, Donna Nofziger

Start Date

18-11-2017 12:30 PM

End Date

18-11-2017 1:30 PM

Location

BSC-Ursa Minor 74

Session

Poster 2

Type of Presentation

Poster

Subject Area

biological_agricultural_sciences

Abstract

The Chumash Native Americans used many native plants as a treatment for cuts and wounds. Angiogenesis, the formation of new blood vessels, plays an essential role in wound healing and is characterized by increased endothelial cell proliferation and migration. Mexican elderberry is a traditional Chumash medicinal plant used for treating cuts and wounds and has been previously shown in our lab to cause an increase in proliferation of Human Umbilical Vascular Endothelial Cells (HUVECs). Based on literature reports, two possible compounds responsible for this increase are rutin and chlorogenic acid. Both of these compounds were identified in our prior extracts of Mexican elderberry and we hypothesized that treatment of HUVECS with rutin and chlorogenic acid would cause a change in the proliferation of these cells. We performed cell proliferation assays in 96 well plates with cells passaged between one and six times, cultured in EGM-2 media and incubated at 37∘C with 5% CO2. Then, we added WST-8 dye to each well twenty-four hours after adding the treatments to quantify the number of live cells. Upon analysis, we found that treatment with rutin and chlorogenic acid individually or in combination did not consistently increase proliferation. Our data failed to support our hypothesis and suggest that rutin and chlorogenic acid are not responsible for the increase in proliferation previously observed with elderberry extract.

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Nov 18th, 12:30 PM Nov 18th, 1:30 PM

Proliferative Effects of Rutin and Chlorogenic Acid on HUVECs

BSC-Ursa Minor 74

The Chumash Native Americans used many native plants as a treatment for cuts and wounds. Angiogenesis, the formation of new blood vessels, plays an essential role in wound healing and is characterized by increased endothelial cell proliferation and migration. Mexican elderberry is a traditional Chumash medicinal plant used for treating cuts and wounds and has been previously shown in our lab to cause an increase in proliferation of Human Umbilical Vascular Endothelial Cells (HUVECs). Based on literature reports, two possible compounds responsible for this increase are rutin and chlorogenic acid. Both of these compounds were identified in our prior extracts of Mexican elderberry and we hypothesized that treatment of HUVECS with rutin and chlorogenic acid would cause a change in the proliferation of these cells. We performed cell proliferation assays in 96 well plates with cells passaged between one and six times, cultured in EGM-2 media and incubated at 37∘C with 5% CO2. Then, we added WST-8 dye to each well twenty-four hours after adding the treatments to quantify the number of live cells. Upon analysis, we found that treatment with rutin and chlorogenic acid individually or in combination did not consistently increase proliferation. Our data failed to support our hypothesis and suggest that rutin and chlorogenic acid are not responsible for the increase in proliferation previously observed with elderberry extract.