Presentation Title

Characterization and analysis of integrins on immune cells found in canine carcinomas.

Faculty Mentor

Chad Barber PhD.

Start Date

18-11-2017 12:30 PM

End Date

18-11-2017 1:30 PM

Location

BSC-Ursa Minor 63

Session

Poster 2

Type of Presentation

Poster

Subject Area

biological_agricultural_sciences

Abstract

The purpose of this study is to identify and analyze integrins found on cytotoxic T cells, macrophages, and tumor cells present in canine tumors. Additionally, we aim to identify roles of these integrins in immune cells as well as other cells found in the tumor. This area of research has been studied extensively in humans, however, much of this is unknown with regards to canine species. Based on previous studies, I predict that the canine integrin alphaLbeta2 will be found on cytotoxic T cells and the integrin alphaMbeta2 will be found on macrophages in canine primary tumors analyzed. Primary methods for this study include cell culture and flow cytometry. Tumors received from veterinarians are rinsed with basal media and disaggregated; half the cells are seeded for cell culture. The remaining half are used for flow cytometry. We stained the cells with fluorescently-labeled antibodies that bind to specific integrins. The cells are run through the flow cytometer to detect integrin expression of the cells. We have been using antibodies specific to canine species, but also some that are cross reactive for canine. We have seen expression of the alphaL subunit with two tumor samples. In previous studies, we have found expression of the alpha6 subunit on the canine cancer line, D17. We aim to perform inhibition assays with integrin blocking antibodies or peptides in vitro to assess the role in survival, migration or adhesion in the tumor microenvironment. In previous studies with the D17 cancer line, we found that the small peptide RGD, an adhesion inhibitor, prevented cells from growing. By learning more about the integrins expressed on cytotoxic T cells and macrophages in canine tumors, as well as on the tumor cells, we can make predictions about the role that the integrins play in cancer progression.

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Nov 18th, 12:30 PM Nov 18th, 1:30 PM

Characterization and analysis of integrins on immune cells found in canine carcinomas.

BSC-Ursa Minor 63

The purpose of this study is to identify and analyze integrins found on cytotoxic T cells, macrophages, and tumor cells present in canine tumors. Additionally, we aim to identify roles of these integrins in immune cells as well as other cells found in the tumor. This area of research has been studied extensively in humans, however, much of this is unknown with regards to canine species. Based on previous studies, I predict that the canine integrin alphaLbeta2 will be found on cytotoxic T cells and the integrin alphaMbeta2 will be found on macrophages in canine primary tumors analyzed. Primary methods for this study include cell culture and flow cytometry. Tumors received from veterinarians are rinsed with basal media and disaggregated; half the cells are seeded for cell culture. The remaining half are used for flow cytometry. We stained the cells with fluorescently-labeled antibodies that bind to specific integrins. The cells are run through the flow cytometer to detect integrin expression of the cells. We have been using antibodies specific to canine species, but also some that are cross reactive for canine. We have seen expression of the alphaL subunit with two tumor samples. In previous studies, we have found expression of the alpha6 subunit on the canine cancer line, D17. We aim to perform inhibition assays with integrin blocking antibodies or peptides in vitro to assess the role in survival, migration or adhesion in the tumor microenvironment. In previous studies with the D17 cancer line, we found that the small peptide RGD, an adhesion inhibitor, prevented cells from growing. By learning more about the integrins expressed on cytotoxic T cells and macrophages in canine tumors, as well as on the tumor cells, we can make predictions about the role that the integrins play in cancer progression.