Presentation Title

Characterization of CHIZ Cells as a Unique Cell Population in Drosophila Blood Cell Development

Faculty Mentor

Utpal Banerjee

Start Date

18-11-2017 12:30 PM

End Date

18-11-2017 1:30 PM

Location

BSC-Ursa Minor 80

Session

Poster 2

Type of Presentation

Poster

Subject Area

biological_agricultural_sciences

Abstract

The Drosophila blood system represents a genetically tractable model to better understand human blood development. Previous research efforts have postulated that there exists a population of cells located between the mature and progenitor cell zones in the lymph gland, the organ in which blood cells develop in the larvae. These Combined Hematopoietic Intermediate Zone (CHIZ) cells express both mature and progenitor cell markers at lower levels than true mature and progenitor cells. CHIZ cells might have special functions that are not characteristic of solely the mature or progenitor cells. This study characterizes these cells to better understand their identity and function. We concluded that CHIZ cells mostly do not colocalize with mature hemocytes like plasmatocytes and crystal cells in the lymph gland. Genetic ablation of lymph gland CHIZ cells results in reduced CHIZ cells, but does not dramatically change crystal cell number. Experiments analyzing circulating hemocytes revealed that CHIZ cells make up only about 1-5% of circulating hemocytes. We also found that most CHIZ cells in circulation colocalize with a mature plasmatocyte marker. These findings suggest that the CHIZ cells from the lymph gland might have a distinct function from the CHIZ cells in circulation, and that CHIZ cells might not be necessary for establishing the crystal cell population. As CHIZ cells are a key transitional cell population where progenitor cells decide which differentiated cell to become, and as pathways that influence differentiation are most likely acting specifically in these cells, efforts to understand CHIZ cells could lead to breakthroughs in blood development-related research.

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Nov 18th, 12:30 PM Nov 18th, 1:30 PM

Characterization of CHIZ Cells as a Unique Cell Population in Drosophila Blood Cell Development

BSC-Ursa Minor 80

The Drosophila blood system represents a genetically tractable model to better understand human blood development. Previous research efforts have postulated that there exists a population of cells located between the mature and progenitor cell zones in the lymph gland, the organ in which blood cells develop in the larvae. These Combined Hematopoietic Intermediate Zone (CHIZ) cells express both mature and progenitor cell markers at lower levels than true mature and progenitor cells. CHIZ cells might have special functions that are not characteristic of solely the mature or progenitor cells. This study characterizes these cells to better understand their identity and function. We concluded that CHIZ cells mostly do not colocalize with mature hemocytes like plasmatocytes and crystal cells in the lymph gland. Genetic ablation of lymph gland CHIZ cells results in reduced CHIZ cells, but does not dramatically change crystal cell number. Experiments analyzing circulating hemocytes revealed that CHIZ cells make up only about 1-5% of circulating hemocytes. We also found that most CHIZ cells in circulation colocalize with a mature plasmatocyte marker. These findings suggest that the CHIZ cells from the lymph gland might have a distinct function from the CHIZ cells in circulation, and that CHIZ cells might not be necessary for establishing the crystal cell population. As CHIZ cells are a key transitional cell population where progenitor cells decide which differentiated cell to become, and as pathways that influence differentiation are most likely acting specifically in these cells, efforts to understand CHIZ cells could lead to breakthroughs in blood development-related research.