Presentation Title

The Role of the Cell Wall in the Resistance of Bacillus Subtilis to the Complement System

Presenter Information

Genevieve HerleFollow

Faculty Mentor

Dr. Ben Aronson

Start Date

18-11-2017 12:30 PM

End Date

18-11-2017 1:30 PM

Location

BSC-Ursa Minor 44

Session

Poster 2

Type of Presentation

Poster

Subject Area

biological_agricultural_sciences

Abstract

Gram positive bacteria are resistant to the killing effects of the complement system. Bacillus subtilis was used as a subject to identify the components of gram positive bacterial cell walls responsible for complement resistance. Protoplasts were created from B. subtilis cells and treated with complement proteins. Complement resistance of the protoplasted cells was determined. Preliminary results indicate a dependence on the peptidoglycan layer for complement resistance.

Summary of research results to be presented

Protoplasted B. subtilis cells showed increased resistance to human serum compared to normal cells. Thus indicates that the thick peptidoglycan layer in the Gram positive B. subtilis cells is necessary for their serum resistance. The protoplasts did not show any sensitivity to complement proteins specifically, only to serum in general. It also appears that B. subtilis cells grown at 30°C instead of 37°C have increased sensitivity to serum. Investigation of effect of growth temperature on serum sensitivity is ongoing.

This document is currently not available here.

Share

COinS
 
Nov 18th, 12:30 PM Nov 18th, 1:30 PM

The Role of the Cell Wall in the Resistance of Bacillus Subtilis to the Complement System

BSC-Ursa Minor 44

Gram positive bacteria are resistant to the killing effects of the complement system. Bacillus subtilis was used as a subject to identify the components of gram positive bacterial cell walls responsible for complement resistance. Protoplasts were created from B. subtilis cells and treated with complement proteins. Complement resistance of the protoplasted cells was determined. Preliminary results indicate a dependence on the peptidoglycan layer for complement resistance.