Presentation Title

Characterization of Dog, Cat, Guinea Pig, and Human IAPP toxicity in HeLa Cells

Faculty Mentor

Luiza Nogaj PhD

Start Date

18-11-2017 12:30 PM

End Date

18-11-2017 1:30 PM

Location

BSC-Ursa Minor 50

Session

Poster 2

Type of Presentation

Poster

Subject Area

biological_agricultural_sciences

Abstract

Type 2 diabetes is one of the leading causes of death in the U.S. Type 2 Diabetes is the most generic form of diabetes, where your body suffers from insulin resistance. Islet Amyloid Polypeptide (IAPP) is a regulatory peptide which inhibits glucagon and insulin secretion. The aggregation of IAPP is present in pancreatic islet amyloid deposits seen especially in Type 2 diabetes in humans and other mammalian species. Our objective was to see how the changes in IAPP sequence correlates to the toxicity of IAPP on mammalian cells. Here, we focus on dog, cat, and guinea pig IAPP. Molecular techniques such as MTT assay and LDH Cytotoxicity assay were used to test the effect animal IAPP has on HeLa cells. MTT assay results showed that the animal IAPP concentrations had no significant effect on mammalian cell viability while the human IAPP was consistently toxic to the cells. Cell viability percentages stayed relatively constant despite increasing IAPP concentration. In comparison, human IAPP showed a decreasing cell viability trend with increasing IAPP concentration. LDH Cytotoxicity assay results showed that percent toxicity increased with increasing IAPP concentrations for human, cat and guinea pig. Dog IAPP cell toxicity stayed constant as IAPP concentration increased. Further experiments are necessary to determine a correlation between these specific animal IAPPs and cell toxicity.

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Nov 18th, 12:30 PM Nov 18th, 1:30 PM

Characterization of Dog, Cat, Guinea Pig, and Human IAPP toxicity in HeLa Cells

BSC-Ursa Minor 50

Type 2 diabetes is one of the leading causes of death in the U.S. Type 2 Diabetes is the most generic form of diabetes, where your body suffers from insulin resistance. Islet Amyloid Polypeptide (IAPP) is a regulatory peptide which inhibits glucagon and insulin secretion. The aggregation of IAPP is present in pancreatic islet amyloid deposits seen especially in Type 2 diabetes in humans and other mammalian species. Our objective was to see how the changes in IAPP sequence correlates to the toxicity of IAPP on mammalian cells. Here, we focus on dog, cat, and guinea pig IAPP. Molecular techniques such as MTT assay and LDH Cytotoxicity assay were used to test the effect animal IAPP has on HeLa cells. MTT assay results showed that the animal IAPP concentrations had no significant effect on mammalian cell viability while the human IAPP was consistently toxic to the cells. Cell viability percentages stayed relatively constant despite increasing IAPP concentration. In comparison, human IAPP showed a decreasing cell viability trend with increasing IAPP concentration. LDH Cytotoxicity assay results showed that percent toxicity increased with increasing IAPP concentrations for human, cat and guinea pig. Dog IAPP cell toxicity stayed constant as IAPP concentration increased. Further experiments are necessary to determine a correlation between these specific animal IAPPs and cell toxicity.