Presentation Title

Cultivation and Identification of Micrococcus Bacterium

Faculty Mentor

Hector Valenzuela

Start Date

18-11-2017 12:30 PM

End Date

18-11-2017 1:30 PM

Location

BSC-Ursa Minor 59

Session

Poster 2

Type of Presentation

Poster

Subject Area

biological_agricultural_sciences

Abstract

In microbiology, the classification of microorganisms is a foundational step. This study focused on the classification of an unknown bacteria. The intended aim of this experiment was to classify this particular microorganism on the genus level using a series of identification techniques. These methods include, Gram Staining, Spore Staining, Catalase testing, motility testing, NaCl growth testing and PCR. Our results of the aforementioned techniques were the following: Gram positive bacteria, Spore staining resulted in no spores, Positive Catalase test, Positive Motility Test. NaCl growth testing resulted in 5% as opposed to no growth in media with 10% NaCl. The results collectively indicated that the unknown bacteria were of the Micrococcus genus. Confirmation of the bacteria was done by MLUT_RS11615 gene-specific PCR amplification.

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Nov 18th, 12:30 PM Nov 18th, 1:30 PM

Cultivation and Identification of Micrococcus Bacterium

BSC-Ursa Minor 59

In microbiology, the classification of microorganisms is a foundational step. This study focused on the classification of an unknown bacteria. The intended aim of this experiment was to classify this particular microorganism on the genus level using a series of identification techniques. These methods include, Gram Staining, Spore Staining, Catalase testing, motility testing, NaCl growth testing and PCR. Our results of the aforementioned techniques were the following: Gram positive bacteria, Spore staining resulted in no spores, Positive Catalase test, Positive Motility Test. NaCl growth testing resulted in 5% as opposed to no growth in media with 10% NaCl. The results collectively indicated that the unknown bacteria were of the Micrococcus genus. Confirmation of the bacteria was done by MLUT_RS11615 gene-specific PCR amplification.