Presentation Title

Enzyme Encapsulation in Metal Organic Frameworks for Increased Stability

Faculty Mentor

Yangyang Liu

Start Date

18-11-2017 12:30 PM

End Date

18-11-2017 1:30 PM

Location

BSC-Ursa Minor 132

Session

Poster 2

Type of Presentation

Poster

Subject Area

physical_mathematical_sciences

Abstract

Glucose oxidase (GOx) is an enzyme that catalyzes the oxidation of glucose to gluconic acid and is primarily used to detect glucose activity. However, GOx is only stable at low temperatures and solvents with pH at or near 7.0. The encapsulation of GOx in iron-based metal-organic frameworks (MOFs) was hypothesized to increase enzyme stability in a greater range of conditions. MOFs are high surface area, permanent porosity, crystalline materials composed of metal ions and organic linkers. The MOF Zr PCN-222(Fe) was selected for encapsulation due to its large pore size (3.7 nm) and high thermal and chemical stability. GOx was successfully encapsulated by suspending Zr PCN-222(Fe) in a range of concentrations of aqueous GOx solution to ensure maximum loading. It was found that enzyme encapsulation is proportional to the concentration of the solution used, with maximum loading capacity at 0.936 mg/mL solution of GOx. The maximum loading capacity of GOx within Zr PCN-222(Fe) was shown to be 3.48% by weight using ultraviolet-visible spectroscopy (UV-vis). UV-vis data taken for each sample was used to determine the loading time for maximum GOx encapsulation, which was found to be one hour. The adsorption of GOx within Zr PCN-222(Fe) remains constant beyond a one hour increment. Future research will examine the thermodynamic and chemical stability of encapsulated GOx using quantitative measures, as well as investigate whether enzyme encapsulation using MOFs with pore sizes larger than 3.7nm will increase the observed maximum loading capacity.

The funding for the Cal State LA LSAMP-BD Cohort XIII program is provided by the National Science Foundation under Grant # HRD-1602210.

Summary of research results to be presented

GOx was successfully encapsulated by suspending Zr PCN-222(Fe) in a range of concentrations of aqueous GOx solution to ensure maximum loading. It was found that enzyme encapsulation is proportional to the concentration of the solution used, with maximum loading capacity at 0.936 mg/mL solution of GOx. The maximum loading capacity of GOx within Zr PCN-222(Fe) was shown to be 3.48% by weight using ultraviolet-visible spectroscopy (UV-vis). UV-vis data taken for each sample was used to determine the loading time for maximum GOx encapsulation, which was found to be one hour. The adsorption of GOx within Zr PCN-222(Fe) remains constant beyond a one hour increment.

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Nov 18th, 12:30 PM Nov 18th, 1:30 PM

Enzyme Encapsulation in Metal Organic Frameworks for Increased Stability

BSC-Ursa Minor 132

Glucose oxidase (GOx) is an enzyme that catalyzes the oxidation of glucose to gluconic acid and is primarily used to detect glucose activity. However, GOx is only stable at low temperatures and solvents with pH at or near 7.0. The encapsulation of GOx in iron-based metal-organic frameworks (MOFs) was hypothesized to increase enzyme stability in a greater range of conditions. MOFs are high surface area, permanent porosity, crystalline materials composed of metal ions and organic linkers. The MOF Zr PCN-222(Fe) was selected for encapsulation due to its large pore size (3.7 nm) and high thermal and chemical stability. GOx was successfully encapsulated by suspending Zr PCN-222(Fe) in a range of concentrations of aqueous GOx solution to ensure maximum loading. It was found that enzyme encapsulation is proportional to the concentration of the solution used, with maximum loading capacity at 0.936 mg/mL solution of GOx. The maximum loading capacity of GOx within Zr PCN-222(Fe) was shown to be 3.48% by weight using ultraviolet-visible spectroscopy (UV-vis). UV-vis data taken for each sample was used to determine the loading time for maximum GOx encapsulation, which was found to be one hour. The adsorption of GOx within Zr PCN-222(Fe) remains constant beyond a one hour increment. Future research will examine the thermodynamic and chemical stability of encapsulated GOx using quantitative measures, as well as investigate whether enzyme encapsulation using MOFs with pore sizes larger than 3.7nm will increase the observed maximum loading capacity.

The funding for the Cal State LA LSAMP-BD Cohort XIII program is provided by the National Science Foundation under Grant # HRD-1602210.