Presentation Title

Identification and Annotation of Protein Orthology in Drosophila elegans

Presenter Information

Eduardo CruzFollow

Faculty Mentor

Alexa Sawa

Start Date

18-11-2017 12:30 PM

End Date

18-11-2017 1:30 PM

Location

BSC-Ursa Minor 68

Session

Poster 2

Type of Presentation

Poster

Subject Area

biological_agricultural_sciences

Abstract

Evaluation of possible genetic coding sequence conservation across two species of Drosophila using comparative genomics. Identification and subsequent annotation of conserved protein orthologues between D. melanogaster (Dmel) and D. elegans (Dele) was completed. Using Genscan software to identify possible coding sequences in a 60,000bp contig of Dele. Predicted coding genetic sequences were identified and screened using Flybase and Blastx software. Protein predictions with strong conservation to known proteins in Dmel were annotated using amino acid alignments constructed with NCBI Blast alignment tool. The results of these assessments strongly indicate evidence supporting that seven predicted proteins found in the contig of Dele are conserved orthologous found in Dmel. The research goal of this project is to further the understanding of conserved hetero-chromatin structure and phenotypic expression within the species of Drosophila.

Summary of research results to be presented

Flybase results provided a baseline for annotation of all predicated genes with the exception of predicted gene 37.1 which returned no data from Flybase. The six Genscan protein predictions produced seven orthologs found in Dmel, with results for predictions 37.2 - 37.3 sharing three protein orthologs found in Dmel. Predictions for Genscan 37.2, 37.3, 37.5, and 37.6 were successfully annotated. Results for Genscan predictions 37.2, 37.4, and 37.7 showed anomalies during annotation. Genscan 37.2 indicated the presence of Oct-TyrR, a protein found in Dmel with 2 isoforms, each having identical coding sequences and three exons. Annotation in Dele indicated strong conservation of the coding sequence, however exon structure was not well conserved. Genscan 37.4 indicated the presence of CG7133, a protein win only one exon and a single isoform. Conservation however is low, with only an Identity of 36.2%, and 26.9% Gap score. Dot plot analysis presented several large horizontal and vertical gaps in coverage, indicating breaks in protein conservation. Genscan 37.7 posed the greatest anomalous annotation, Flybase returned hits for several genes belonging to the Cytochrome P450. The strongest hit using the Genscan prediction model resulting in an E-value of 2.2e-180, corresponding to protein Cyp313a4 in Dmel. Cyp313a4 is a gene with 7 exons and only 1 isoform, most notably, Gene Record Finder lists this ortholog as present on Chromosome 3R of Dele

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Nov 18th, 12:30 PM Nov 18th, 1:30 PM

Identification and Annotation of Protein Orthology in Drosophila elegans

BSC-Ursa Minor 68

Evaluation of possible genetic coding sequence conservation across two species of Drosophila using comparative genomics. Identification and subsequent annotation of conserved protein orthologues between D. melanogaster (Dmel) and D. elegans (Dele) was completed. Using Genscan software to identify possible coding sequences in a 60,000bp contig of Dele. Predicted coding genetic sequences were identified and screened using Flybase and Blastx software. Protein predictions with strong conservation to known proteins in Dmel were annotated using amino acid alignments constructed with NCBI Blast alignment tool. The results of these assessments strongly indicate evidence supporting that seven predicted proteins found in the contig of Dele are conserved orthologous found in Dmel. The research goal of this project is to further the understanding of conserved hetero-chromatin structure and phenotypic expression within the species of Drosophila.