Presentation Title

Analysis of the Biochemical Pathway for Biofuel Production using Clostridium Strains

Faculty Mentor

Wei-Jen Lin PhD, Gregory Barding PhD

Start Date

18-11-2017 12:30 PM

End Date

18-11-2017 1:30 PM

Location

BSC-Ursa Minor 75

Session

Poster 2

Type of Presentation

Poster

Subject Area

biological_agricultural_sciences

Abstract

Humans have contributed to the deterioration of the environment; and as climate change becomes more of a threat to our way of life; it is imperative that sustainable practices are established. The environmental impact of fossil fuel consumption is taking a toll on the ozone layer, leading to climate change that could potentially be irreversible. In an effort to generate a sustainable and environmentally conscious alternative to fossil fuels, Clostridium beijerinckii, an anaerobic spore forming bacteria is being studied and analyzed for its production of biofuels. Five C. beijerinckii isolates were analyzed for the presence of acetone-butanol-ethanol (ABE) fermentation pathway using gene specific PCR primers. The yields of the solvents were analyzed through Nuclear Magnetic Resonance (NMR). Our results indicated that four out of five strains harbor the genes for the ABE fermentation pathway, including acetoacetyl decarboxylase, butanol dehydrogenase, and alcohol dehydrogenase, which was supported by the significant yields of acetone, butanol and ethanol. One strain, L34B, demonstrated the lack of the ABE genes as well as the products. However, the butyrate yield was high in L34B, which may imply a divergent of metabolic pathway in different strains. The production of ABE products in these C. beijerinckii strains were further optimized in scale-up containers. Our unique high butanol producing C. beijerinckii strains may provide potential candidates for future large scale production of butanol as an alternative biofuel source.

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Nov 18th, 12:30 PM Nov 18th, 1:30 PM

Analysis of the Biochemical Pathway for Biofuel Production using Clostridium Strains

BSC-Ursa Minor 75

Humans have contributed to the deterioration of the environment; and as climate change becomes more of a threat to our way of life; it is imperative that sustainable practices are established. The environmental impact of fossil fuel consumption is taking a toll on the ozone layer, leading to climate change that could potentially be irreversible. In an effort to generate a sustainable and environmentally conscious alternative to fossil fuels, Clostridium beijerinckii, an anaerobic spore forming bacteria is being studied and analyzed for its production of biofuels. Five C. beijerinckii isolates were analyzed for the presence of acetone-butanol-ethanol (ABE) fermentation pathway using gene specific PCR primers. The yields of the solvents were analyzed through Nuclear Magnetic Resonance (NMR). Our results indicated that four out of five strains harbor the genes for the ABE fermentation pathway, including acetoacetyl decarboxylase, butanol dehydrogenase, and alcohol dehydrogenase, which was supported by the significant yields of acetone, butanol and ethanol. One strain, L34B, demonstrated the lack of the ABE genes as well as the products. However, the butyrate yield was high in L34B, which may imply a divergent of metabolic pathway in different strains. The production of ABE products in these C. beijerinckii strains were further optimized in scale-up containers. Our unique high butanol producing C. beijerinckii strains may provide potential candidates for future large scale production of butanol as an alternative biofuel source.