Presentation Title

Identification of an Unknown Bacteria Genus: Pseudomonas

Faculty Mentor

Hector Valenzuela

Start Date

18-11-2017 12:30 PM

End Date

18-11-2017 1:30 PM

Location

BSC-Ursa Minor 40

Session

Poster 2

Type of Presentation

Poster

Subject Area

biological_agricultural_sciences

Abstract

The purpose of this experiment was to develop and confirm the techniques needed to identify an unknown bacteria. The series of techniques which were performed in the experiment included tests of morphology (observation of bacterial cell shape, size, motility, and gram staining), culture (observation of the bacteria grown in varying environments and conditions based on different agar plates), and physiology (catalase testing / PCR / species-specific gene confirmation). A blind study using an unknown bacteria was utilized for these experiments.The administered tests on the unknown bacteria produced results that showed that the bacteria was gram-negative, rod-shaped, motile, aerobic, and catalase positive with the specific gene utilized being putative fatty acid desaturase. The bacteria was also able to grow in low salt concentrated agar plates. This series of observations lead to the conclusion that the given, unknown bacteria genus was Pseudomonas, a genus of rod shaped, gram-negative, aerobic bacteria commonly found in soil, plants, water, and human beings that, since it’s discovery, remains extremely prominent for scientific research in microbiology.

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Nov 18th, 12:30 PM Nov 18th, 1:30 PM

Identification of an Unknown Bacteria Genus: Pseudomonas

BSC-Ursa Minor 40

The purpose of this experiment was to develop and confirm the techniques needed to identify an unknown bacteria. The series of techniques which were performed in the experiment included tests of morphology (observation of bacterial cell shape, size, motility, and gram staining), culture (observation of the bacteria grown in varying environments and conditions based on different agar plates), and physiology (catalase testing / PCR / species-specific gene confirmation). A blind study using an unknown bacteria was utilized for these experiments.The administered tests on the unknown bacteria produced results that showed that the bacteria was gram-negative, rod-shaped, motile, aerobic, and catalase positive with the specific gene utilized being putative fatty acid desaturase. The bacteria was also able to grow in low salt concentrated agar plates. This series of observations lead to the conclusion that the given, unknown bacteria genus was Pseudomonas, a genus of rod shaped, gram-negative, aerobic bacteria commonly found in soil, plants, water, and human beings that, since it’s discovery, remains extremely prominent for scientific research in microbiology.