Presentation Title

Identification of Nicotiana glutinosa genes that are differentially regulated in defense responses against Polerovirus

Faculty Mentor

Melanie Sacco

Start Date

18-11-2017 12:30 PM

End Date

18-11-2017 1:30 PM

Location

BSC-Ursa Minor 38

Session

Poster 2

Type of Presentation

Poster

Subject Area

biological_agricultural_sciences

Abstract

Plants defend themselves against infectious pathogens either through pre-existing defenses, such as physical barriers that prevent infections, or through inducible defenses that operate at the molecular level through immune receptors that are activated by pathogen elicitors. Examples of inducible responses to pathogens include extreme resistance (ER) in which the pathogen is eliminated without cell death, and hypersensitive response (HR), which limits the pathogen to the infected area by local programmed cell death. When infected with different members of the Polerovirus genus, two accessions of Nioctiana glutinosa displayed either HR or ER. N. glutinosa accession TW59 exhibited HR when infected with Turnip yellows virus (TuYV) as well as Potato leaf roll virus (PLRV). Accession TW61 exhibited HR only when infected by PLRV, but showed ER when infected by TuYV. RNA was isolated from both uninfected leaves and leaves infected by TuYV or PLRV from the two accessions for analysis by Next Generation RNA-sequencing (RNA-seq) to determine the gene expression changes in response to each virus. RNA from infected tissue was collected thirty-six hours post infiltration before the visible onset of cell death. We hypothesize that there will be specific changes in gene expression associated with ER versus resistance accompanied by HR, as well as some shared gene expression changes for these responses. Genes of interest will be further tested for differential expression through real-time reverse transcription-quantitative polymerase chain reaction (RT-qPCR). We aim to identify candidate genes with overlapping roles in ER and HR or with unique roles in one defense pathway in order to obtain a better understanding of these separate but intersecting plant defense responses.

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Nov 18th, 12:30 PM Nov 18th, 1:30 PM

Identification of Nicotiana glutinosa genes that are differentially regulated in defense responses against Polerovirus

BSC-Ursa Minor 38

Plants defend themselves against infectious pathogens either through pre-existing defenses, such as physical barriers that prevent infections, or through inducible defenses that operate at the molecular level through immune receptors that are activated by pathogen elicitors. Examples of inducible responses to pathogens include extreme resistance (ER) in which the pathogen is eliminated without cell death, and hypersensitive response (HR), which limits the pathogen to the infected area by local programmed cell death. When infected with different members of the Polerovirus genus, two accessions of Nioctiana glutinosa displayed either HR or ER. N. glutinosa accession TW59 exhibited HR when infected with Turnip yellows virus (TuYV) as well as Potato leaf roll virus (PLRV). Accession TW61 exhibited HR only when infected by PLRV, but showed ER when infected by TuYV. RNA was isolated from both uninfected leaves and leaves infected by TuYV or PLRV from the two accessions for analysis by Next Generation RNA-sequencing (RNA-seq) to determine the gene expression changes in response to each virus. RNA from infected tissue was collected thirty-six hours post infiltration before the visible onset of cell death. We hypothesize that there will be specific changes in gene expression associated with ER versus resistance accompanied by HR, as well as some shared gene expression changes for these responses. Genes of interest will be further tested for differential expression through real-time reverse transcription-quantitative polymerase chain reaction (RT-qPCR). We aim to identify candidate genes with overlapping roles in ER and HR or with unique roles in one defense pathway in order to obtain a better understanding of these separate but intersecting plant defense responses.