Presentation Title

Mutation of ABC Transporter Protein plays a role in the expression of EPS and motility in Paraburkholderia unamae

Faculty Mentor

Shelley Thai, Ph.D.

Start Date

18-11-2017 12:30 PM

End Date

18-11-2017 1:30 PM

Location

BSC-Ursa Minor 79

Session

Poster 2

Type of Presentation

Poster

Subject Area

biological_agricultural_sciences

Abstract

Paraburkholderia unamae is an endosymbiont of plants, such as maize and tomato, and produces many plant promoting effects which have great agricultural importance. These include nitrogen fixation and ACC deaminase activity. Motility and exopolysaccharide (EPS) production are the two key characteristics which allow these relationships to be formed. In order to study the genes involved in these phenotypes, a suicide vector that carries a hyperactive transposon Tn5-RL27 was used to create random mutation in the genome and the resulting mutants were screened for the desired phenotypes: over EPS production (mucoidy appearance) and reduced or impaired motility. Four mutants contained Tn5 disruptions in different regions of the ATP Binding Cassette gene that codes for an ABC transporter protein. The mutation of this gene had a dual effect on both EPS and motility. EPS production was increased and motility was reduced by 60.14%, 34.70%, 27.53%, and 16.67% in all four mutants in comparison to wild-type. ABC transporter systems couple the energy of ATP hydrolysis to import and export a wide variety of biological molecules and solutes through transmembrane transport. We believe the ABC transporter protein plays a dual role in motility and EPS production. The ABC transporter protein may be directly involved in motility of P. unamae by transporting the ions required to create an ion gradient at the plasma membrane which in turn drives the flagellar filament to produce motility. Additionally, ABC transporter proteins may play an indirect role in the production of EPS by regulating the genes involved in EPS expression. Further studies are needed to elucidate its role in these processes.

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Nov 18th, 12:30 PM Nov 18th, 1:30 PM

Mutation of ABC Transporter Protein plays a role in the expression of EPS and motility in Paraburkholderia unamae

BSC-Ursa Minor 79

Paraburkholderia unamae is an endosymbiont of plants, such as maize and tomato, and produces many plant promoting effects which have great agricultural importance. These include nitrogen fixation and ACC deaminase activity. Motility and exopolysaccharide (EPS) production are the two key characteristics which allow these relationships to be formed. In order to study the genes involved in these phenotypes, a suicide vector that carries a hyperactive transposon Tn5-RL27 was used to create random mutation in the genome and the resulting mutants were screened for the desired phenotypes: over EPS production (mucoidy appearance) and reduced or impaired motility. Four mutants contained Tn5 disruptions in different regions of the ATP Binding Cassette gene that codes for an ABC transporter protein. The mutation of this gene had a dual effect on both EPS and motility. EPS production was increased and motility was reduced by 60.14%, 34.70%, 27.53%, and 16.67% in all four mutants in comparison to wild-type. ABC transporter systems couple the energy of ATP hydrolysis to import and export a wide variety of biological molecules and solutes through transmembrane transport. We believe the ABC transporter protein plays a dual role in motility and EPS production. The ABC transporter protein may be directly involved in motility of P. unamae by transporting the ions required to create an ion gradient at the plasma membrane which in turn drives the flagellar filament to produce motility. Additionally, ABC transporter proteins may play an indirect role in the production of EPS by regulating the genes involved in EPS expression. Further studies are needed to elucidate its role in these processes.