Presentation Title

Selection of DNA Aptamers Targeting Listeria p60

Faculty Mentor

Junjun Liu

Start Date

18-11-2017 2:15 PM

End Date

18-11-2017 3:15 PM

Location

BSC-Ursa Minor 90

Session

Poster 3

Type of Presentation

Poster

Subject Area

biological_agricultural_sciences

Abstract

Selection of DNA Aptamers Targeting Listeria p60

Shawnee Angeloni, Junjun Liu

Dept. of Biological Sciences, Cal Poly Pomona

Listeria monocytogenes is a facultative anaerobic bacterium, which causes the infection listeriosis and is one of the most virulent foodborne pathogens. About 30% of foodborne listeriosis infections in high-risk individuals may be fatal. It is therefore very important to develop rapid and effective methods for L. monocytogenes test to protect human health and avoid costly food recalls. p60 is an extracellular invasion associated protein (iap) secreted by Listeria species in large quantities, which makes it an ideal target for the detection of L. monocytogenes. Antibody-based immunoassays are fast, reproducible, and less sensitive to food interference; therefore are widely used in L. monocytogenes detection. However, due to the nature of antibodies, their binding affinities and quality may vary. Recently, aptamers have emerged as a good alternative to antibodies in diagnostics. Aptamers are stable short single-stranded oligonucleotides or peptides that bind to targets with high affinity and specificity. As they are produced by in vitro synthesis, their quality is more consistent; therefore, they are very suitable for use in food safety tests. In this study, we are using purified recombinant p60 protein as a target to perform systematic evolution of ligands by exponential enrichment (SELEX) to screen for DNA aptamers that specifically target the p60. Previously, this method has been used in the lab to successfully select a DNA aptamer that specifically binds to shiga toxin. The long-term goal of the project is to develop an aptamer-based rapid and specific L. monocytogenes assay kit for use in food safety test.

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Nov 18th, 2:15 PM Nov 18th, 3:15 PM

Selection of DNA Aptamers Targeting Listeria p60

BSC-Ursa Minor 90

Selection of DNA Aptamers Targeting Listeria p60

Shawnee Angeloni, Junjun Liu

Dept. of Biological Sciences, Cal Poly Pomona

Listeria monocytogenes is a facultative anaerobic bacterium, which causes the infection listeriosis and is one of the most virulent foodborne pathogens. About 30% of foodborne listeriosis infections in high-risk individuals may be fatal. It is therefore very important to develop rapid and effective methods for L. monocytogenes test to protect human health and avoid costly food recalls. p60 is an extracellular invasion associated protein (iap) secreted by Listeria species in large quantities, which makes it an ideal target for the detection of L. monocytogenes. Antibody-based immunoassays are fast, reproducible, and less sensitive to food interference; therefore are widely used in L. monocytogenes detection. However, due to the nature of antibodies, their binding affinities and quality may vary. Recently, aptamers have emerged as a good alternative to antibodies in diagnostics. Aptamers are stable short single-stranded oligonucleotides or peptides that bind to targets with high affinity and specificity. As they are produced by in vitro synthesis, their quality is more consistent; therefore, they are very suitable for use in food safety tests. In this study, we are using purified recombinant p60 protein as a target to perform systematic evolution of ligands by exponential enrichment (SELEX) to screen for DNA aptamers that specifically target the p60. Previously, this method has been used in the lab to successfully select a DNA aptamer that specifically binds to shiga toxin. The long-term goal of the project is to develop an aptamer-based rapid and specific L. monocytogenes assay kit for use in food safety test.