Presentation Title

The Effect of Polyphenolic Compounds on the Toxicity of Human IAPP

Faculty Mentor

Dr. Luiza Nogaj

Start Date

18-11-2017 2:15 PM

End Date

18-11-2017 3:15 PM

Location

BSC-Ursa Minor 47

Session

Poster 3

Type of Presentation

Poster

Subject Area

biological_agricultural_sciences

Abstract

In Type II Diabetes pancreatic β-islet cells die due to the aggregation of IAPP (islet amyloid polypeptide, amylin). Finding compounds able to prevent IAPP aggregation could prolong the survival of β-islet cells and slow down the progression of diabetes. EGCG (Epigallocatechin gallate) and PGG (1,2,3,4,6-penta-O-galloyl-β-d-glucose) are compounds that were previously found to inhibit the aggregation of IAPP fibrils, while gallic acid (GA) alone showed no effect. In this study, polyphenols attached to 2-carbon and 3-carbon moieties were examined for their ability to protect the cells from the IAPP toxicity. MTT, cytotoxicity, and apoptosis assays were used to determine the effectiveness of 13 synthetic compounds against IAPP. High cell toxicity and low viability was found in HeLa cells that were in the presence of IAPP, even when treated with compounds. These results conflicted with the atomic force microscopy (AFM) images obtained by our collaborators that showed the ability of some compounds to prevent IAPP fibril aggregation. Our results suggest that the IAPP oligomers unseen in the AFM, not the fibrils, may be the cause of cell death. In the future, we hope to use the Amyloid Fibrils (OC) Antibody in immunohistochemistry and a western blot assay to determine the effects of IAPP on the cells.

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Nov 18th, 2:15 PM Nov 18th, 3:15 PM

The Effect of Polyphenolic Compounds on the Toxicity of Human IAPP

BSC-Ursa Minor 47

In Type II Diabetes pancreatic β-islet cells die due to the aggregation of IAPP (islet amyloid polypeptide, amylin). Finding compounds able to prevent IAPP aggregation could prolong the survival of β-islet cells and slow down the progression of diabetes. EGCG (Epigallocatechin gallate) and PGG (1,2,3,4,6-penta-O-galloyl-β-d-glucose) are compounds that were previously found to inhibit the aggregation of IAPP fibrils, while gallic acid (GA) alone showed no effect. In this study, polyphenols attached to 2-carbon and 3-carbon moieties were examined for their ability to protect the cells from the IAPP toxicity. MTT, cytotoxicity, and apoptosis assays were used to determine the effectiveness of 13 synthetic compounds against IAPP. High cell toxicity and low viability was found in HeLa cells that were in the presence of IAPP, even when treated with compounds. These results conflicted with the atomic force microscopy (AFM) images obtained by our collaborators that showed the ability of some compounds to prevent IAPP fibril aggregation. Our results suggest that the IAPP oligomers unseen in the AFM, not the fibrils, may be the cause of cell death. In the future, we hope to use the Amyloid Fibrils (OC) Antibody in immunohistochemistry and a western blot assay to determine the effects of IAPP on the cells.