Presentation Title

The Hierarchy of Carbon Source Utilization by Sinorhizobium meliloti

Presenter Information

christen hughesFollow

Faculty Mentor

Dr. David Soulsby, Dr. Dan Wacks

Start Date

18-11-2017 2:15 PM

End Date

18-11-2017 3:15 PM

Location

BSC-Ursa Minor 33

Session

Poster 3

Type of Presentation

Poster

Subject Area

physical_mathematical_sciences

Abstract

Sinorhizobium meliloti is a species of bacteria that forms a symbiotic relationship with alfalfa. Throughout their symbiotic relationship, S. meliloti encounters different environments that contain various amounts and types of carbon sources. S. meliloti is known to display a hierarchy of carbon source usage, which has yet to be thoroughly explored. The goal of this project was to determine the hierarchy of carbon using five different carbon sources. To accomplish this goal, we grew S. meliloti in pairs of carbon sources chosen from five possibilities. We then directly studied the consumption of each carbon source using 1H NMR spectroscopy. The five carbon sources that were used were succinate, glucose, sucrose, lactose, and cellobiose. Samples of the cultures were taken periodically and cell growth measured using the optical density at 600 nm. After centrifugation, the supernatant was then examined directly by 1H NMR spectroscopy and the data analyzed using Complete Reduction to Amplitude Frequency Table (CRAFT). By analyzing ten pairs of carbon sources we have determined that S. meliloti prefers succinate to all other carbon sources and glucose to sucrose, cellobiose, or lactose. The hierarchy between sucrose, lactose and cellobiose was more difficult to define, with our data indicating an equal preference between all three.

This document is currently not available here.

Share

COinS
 
Nov 18th, 2:15 PM Nov 18th, 3:15 PM

The Hierarchy of Carbon Source Utilization by Sinorhizobium meliloti

BSC-Ursa Minor 33

Sinorhizobium meliloti is a species of bacteria that forms a symbiotic relationship with alfalfa. Throughout their symbiotic relationship, S. meliloti encounters different environments that contain various amounts and types of carbon sources. S. meliloti is known to display a hierarchy of carbon source usage, which has yet to be thoroughly explored. The goal of this project was to determine the hierarchy of carbon using five different carbon sources. To accomplish this goal, we grew S. meliloti in pairs of carbon sources chosen from five possibilities. We then directly studied the consumption of each carbon source using 1H NMR spectroscopy. The five carbon sources that were used were succinate, glucose, sucrose, lactose, and cellobiose. Samples of the cultures were taken periodically and cell growth measured using the optical density at 600 nm. After centrifugation, the supernatant was then examined directly by 1H NMR spectroscopy and the data analyzed using Complete Reduction to Amplitude Frequency Table (CRAFT). By analyzing ten pairs of carbon sources we have determined that S. meliloti prefers succinate to all other carbon sources and glucose to sucrose, cellobiose, or lactose. The hierarchy between sucrose, lactose and cellobiose was more difficult to define, with our data indicating an equal preference between all three.