Presentation Title

Determination of the Harmful Algae Bloom Microbiome of the Ventura County Transect

Faculty Mentor

Dr. James Harber

Start Date

18-11-2017 2:15 PM

End Date

18-11-2017 3:15 PM

Location

BSC-Ursa Minor 86

Session

Poster 3

Type of Presentation

Poster

Subject Area

biological_agricultural_sciences

Abstract

Harmful algae blooms (HABs) toxins in Ventura County have harmed the shoreline ecosystem and led to economic losses in seafood production, damage to underwater US Navy electronic infrastructure and human disease. HABs are generated population of microorganisms termed mixotrophs increase suddenly to high densities when higher summer ocean temperatures, ample nutrients and water stagnation coincide. This research explored whether a transect of Ventura County ocean water samples can be used to monitor the most predictable habitats for the reservoir of HAB generating organisms by microbiome analysis on an Illumina Myseq next generation sequencing machine. This research confirms, by PCR combined with Sanger DNA sequencing that two mixotroph eukaryotes (Pseudo-nitzschia, Lingulodinium polyedrum) are present in five of the ten Ventura County sites of the transect. The locations of the HAB organisms overlaps with the presence of disease causing Vibrio bacterial population pathogenic for humans and resident in invertebrates (antibiotic resistant Vibrio parahemolyticus and Vibrio kanaloae. Eutrophication of the ocean in Ventura County is proposed to consist of several factors: a shipping port, three extensive inland docks, agricultural/urban effluents and seasonally stagnant rivers. provide ample habitat for HAB organisms and the prey bacteria on the Ventura coastline. Using two sequencing methods in combination yielded a clearer picture of the relationship between eutrophication and the species participating in the origins of HABs for suggesting standard methods for regular sampling of the Ventura ocean ecosystem.

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Nov 18th, 2:15 PM Nov 18th, 3:15 PM

Determination of the Harmful Algae Bloom Microbiome of the Ventura County Transect

BSC-Ursa Minor 86

Harmful algae blooms (HABs) toxins in Ventura County have harmed the shoreline ecosystem and led to economic losses in seafood production, damage to underwater US Navy electronic infrastructure and human disease. HABs are generated population of microorganisms termed mixotrophs increase suddenly to high densities when higher summer ocean temperatures, ample nutrients and water stagnation coincide. This research explored whether a transect of Ventura County ocean water samples can be used to monitor the most predictable habitats for the reservoir of HAB generating organisms by microbiome analysis on an Illumina Myseq next generation sequencing machine. This research confirms, by PCR combined with Sanger DNA sequencing that two mixotroph eukaryotes (Pseudo-nitzschia, Lingulodinium polyedrum) are present in five of the ten Ventura County sites of the transect. The locations of the HAB organisms overlaps with the presence of disease causing Vibrio bacterial population pathogenic for humans and resident in invertebrates (antibiotic resistant Vibrio parahemolyticus and Vibrio kanaloae. Eutrophication of the ocean in Ventura County is proposed to consist of several factors: a shipping port, three extensive inland docks, agricultural/urban effluents and seasonally stagnant rivers. provide ample habitat for HAB organisms and the prey bacteria on the Ventura coastline. Using two sequencing methods in combination yielded a clearer picture of the relationship between eutrophication and the species participating in the origins of HABs for suggesting standard methods for regular sampling of the Ventura ocean ecosystem.