Presentation Title

Development of Extraction and Detection Method for a Chemotherapeutic Drug with Phenytoin in Biological Samples

Faculty Mentor

Karno Ng

Start Date

18-11-2017 2:15 PM

End Date

18-11-2017 3:15 PM

Location

BSC-Ursa Minor 2

Session

Poster 3

Type of Presentation

Poster

Subject Area

physical_mathematical_sciences

Abstract

Among patients with cancer, in particular brain tumors, seizures can become a daily routine in their everyday lives. To counter-act the seizures, an antiepileptic drug such as phenytoin is administered to act as an anticonvulsant. Dexamethasone is classified as a corticosteroid and is commonly used among cancer patients to decrease the amount of swelling around the tumor. Phenytoin and dexamethasone are frequently administrated concurrently to brain cancer patients. A previous study has shown that phenytoin serum concentration decreases when it is administrated concurrently with dexamethasone.[1] Thus, it is important to monitor the concentration of these two drugs in biological samples, in order to ensure that the proper dosages are administrated to the patients.

The purpose of this study is to develop an effective extraction and detection method for dexamethasone and phenytoin. A reverse phase high performance liquid chromatography (HPLC) method with UV/VIS detection has been developed to separate phenytoin and dexamethasone at 219 nm and 241 nm respectively from urine samples. The mobile phase consists of a mixture of 0.01 M KH2PO4, acetonitrile and methanol adjusted to pH 5.6 (48:32:20) and is pumped at a flow rate of 1.0 mL/min. Calibration curves were prepared for phenytoin and dexamethasone (r2 >0.99). An efficient solid phase extraction method with the use of C-18 cartridges for dexamethasone and phenytoin from urine samples was developed. The C-18 solid phase extraction cartridges have hydrophobic selectivity, and do not retain minerals. The percent recovery for phenytoin and dexamethasone is 90.46% and 89.53% respectively.

[1] Thomas E. Lackner, Interaction of Dexamethasone with Phenytoin, Pharmacotherapy 1991; 11(4); 334-347.

Summary of research results to be presented

•r2 value for the calibration curve for phenytoin and dexamethasone are > 0.99 ( excellent linearity) •Percent recovery for phenytoin and dexamethasone are 93.3 % and 84.7 % at the spike level at the 20.2 mg/mL and 27.2 mg/mL •Percent recovery for phenytoin and dexamethasone are 79.2 % and 85.2 % at the spike level at the 4.02 mg/mL and 5.44 mg/mL •Percent recovery is comparable at 2 different spiked concentration.

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Nov 18th, 2:15 PM Nov 18th, 3:15 PM

Development of Extraction and Detection Method for a Chemotherapeutic Drug with Phenytoin in Biological Samples

BSC-Ursa Minor 2

Among patients with cancer, in particular brain tumors, seizures can become a daily routine in their everyday lives. To counter-act the seizures, an antiepileptic drug such as phenytoin is administered to act as an anticonvulsant. Dexamethasone is classified as a corticosteroid and is commonly used among cancer patients to decrease the amount of swelling around the tumor. Phenytoin and dexamethasone are frequently administrated concurrently to brain cancer patients. A previous study has shown that phenytoin serum concentration decreases when it is administrated concurrently with dexamethasone.[1] Thus, it is important to monitor the concentration of these two drugs in biological samples, in order to ensure that the proper dosages are administrated to the patients.

The purpose of this study is to develop an effective extraction and detection method for dexamethasone and phenytoin. A reverse phase high performance liquid chromatography (HPLC) method with UV/VIS detection has been developed to separate phenytoin and dexamethasone at 219 nm and 241 nm respectively from urine samples. The mobile phase consists of a mixture of 0.01 M KH2PO4, acetonitrile and methanol adjusted to pH 5.6 (48:32:20) and is pumped at a flow rate of 1.0 mL/min. Calibration curves were prepared for phenytoin and dexamethasone (r2 >0.99). An efficient solid phase extraction method with the use of C-18 cartridges for dexamethasone and phenytoin from urine samples was developed. The C-18 solid phase extraction cartridges have hydrophobic selectivity, and do not retain minerals. The percent recovery for phenytoin and dexamethasone is 90.46% and 89.53% respectively.

[1] Thomas E. Lackner, Interaction of Dexamethasone with Phenytoin, Pharmacotherapy 1991; 11(4); 334-347.