Presentation Title

Monitoring of Pathogenic E. coli on Petting Zoo Animals at California State University Polytechnic - Pomona

Faculty Mentor

Shelton Murinda, Broc Sandelin

Start Date

18-11-2017 2:15 PM

End Date

18-11-2017 3:15 PM

Location

BSC-Ursa Minor 67

Session

Poster 3

Type of Presentation

Poster

Subject Area

biological_agricultural_sciences

Abstract

Petting zoos are offered at many fairs and children’s programs. Ethanol-based hand sanitizers are predominantly utilized as a hygiene practice after direct contact with the animals. The current hand sanitizer X used at the petting zoo on campus (Cal Poly Pomona) claims “kills 99.99% germs”. This study aims to isolate pathogenic E. coli then examines the bactericidal efficacy of the hand sanitizers on those organisms. By evaluating the effectiveness of the hand sanitizer against E. coli isolates, evidence-based recommendation can be made to the public and the animal handlers to enhance the safety of the petting zoo. We targeted isolations of Shiga toxin-producing E. coli (STEC) strains with the following virulent characteristics: Shiga toxin 1, Shiga toxin 2, enterohemorrhagic hemolysin, and intimin from fecal and trough-water samples obtained from sheep pens in Fall, Winter, and Spring quarters of the academic year 2016-2017. To achieve this, samples were isolated via selective culturing on Chrome Agar, MacConkey Sorbitol, and Chrome Agar STEC plates. PCR and gel electrophoresis were conducted for determination of virulent characteristics of isolates.Total of 4 out of 250 isolates were presumptively positive for some of the STEC virulent factors. This result yields a 1.6% positive from total isolates and 1.8% from the fecal isolates. This appears to suggest that the sheep used as petting animals might carry pathogenic E. coli strains. In the second phase of this study, these isolated and cryopreserved (-20ºC) strains will be used to assess their antimicrobial resistance properties and to test the bactericidal activity of the hand sanitizer X.

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Nov 18th, 2:15 PM Nov 18th, 3:15 PM

Monitoring of Pathogenic E. coli on Petting Zoo Animals at California State University Polytechnic - Pomona

BSC-Ursa Minor 67

Petting zoos are offered at many fairs and children’s programs. Ethanol-based hand sanitizers are predominantly utilized as a hygiene practice after direct contact with the animals. The current hand sanitizer X used at the petting zoo on campus (Cal Poly Pomona) claims “kills 99.99% germs”. This study aims to isolate pathogenic E. coli then examines the bactericidal efficacy of the hand sanitizers on those organisms. By evaluating the effectiveness of the hand sanitizer against E. coli isolates, evidence-based recommendation can be made to the public and the animal handlers to enhance the safety of the petting zoo. We targeted isolations of Shiga toxin-producing E. coli (STEC) strains with the following virulent characteristics: Shiga toxin 1, Shiga toxin 2, enterohemorrhagic hemolysin, and intimin from fecal and trough-water samples obtained from sheep pens in Fall, Winter, and Spring quarters of the academic year 2016-2017. To achieve this, samples were isolated via selective culturing on Chrome Agar, MacConkey Sorbitol, and Chrome Agar STEC plates. PCR and gel electrophoresis were conducted for determination of virulent characteristics of isolates.Total of 4 out of 250 isolates were presumptively positive for some of the STEC virulent factors. This result yields a 1.6% positive from total isolates and 1.8% from the fecal isolates. This appears to suggest that the sheep used as petting animals might carry pathogenic E. coli strains. In the second phase of this study, these isolated and cryopreserved (-20ºC) strains will be used to assess their antimicrobial resistance properties and to test the bactericidal activity of the hand sanitizer X.