Presentation Title

Promising Drug Delivery In Vivo Zebrafish Larvae Using Carbon Dots To Target Calcified Bones

Faculty Mentor

Dr. Roger M. Leblanc, Dr. Zhili Peng

Start Date

18-11-2017 2:15 PM

End Date

18-11-2017 3:15 PM

Location

BSC-Ursa Minor 121

Session

Poster 3

Type of Presentation

Poster

Subject Area

interdisciplinary

Abstract

Carbon nanoparticles, otherwise known as c-dots are a relatively new breakthrough in science with possible prospects in chemical sensing, bio imaging, and other biological applications. Due to their low toxicity levels and various top-down and bottom-up synthesis, c-dot research is high in demand. However, while carbon dots are becoming more prevalent, their generally low quantum yields, in addition to their unclear morphologies have not allowed them to outperform their metal semiconductor nanoparticle rivals. The composition of carbon dots is still widely-unknown and has called for further investigation, although they are mostly comprised of carbon, oxygen, and nitrogen. The use of injection of carbon nanoparticles on 6dpf zebrafish larvae was previously considered in addition to previous work on larvae soaking. Herein, we explored top-down and bottom-up approaches to synthesize black carbon dots, human transferrin conjugate carbon dots, and orange carbon dots. We then soaked zebrafish larvae in various concentrations to target calcified bone thus proving possible applications for drug delivery with human transferrin conjugate c-dots. After treatment, bio imaging occurred on 7dpf-9dpf where fluorescent targeted calcified bones were observed in 3.4 mg/mL, and 5 mg/mL concentrations of black carbon dots in addition to 5 mg/mL transferrin conjugate c-dots. Previous studies using soaking methods have only shown targeted organs and tissue through soaking methods with lower concentrations carbon dot solutions. Using our methods and treatments, we were able to target calcified bones. This was exhibited in soaking that occurred in 3.4, 5, and 5 mg/mL conjugated human transferrin black carbon dot solutions.

Summary of research results to be presented

It is clearly depicted in fig. 1 how auto luminescence in 6dpf control zebrafish larvae differ from the fluorescence observed in larvae soaked in 1, 2, 3.4, and 5 mg/mL concentrations of black carbon dots. Furthermore, it is apparent that further targeting calcified bones is much clearly observed in higher concentrations of black c-dots. However, thanks to fluorescent bioimaging microscopy in fig. 2, we were also able to observe that the orange c-dots did not target the calcified bones. Furthermore, fluorescence was observed in the organs and swim bladder of 7-9dpf larvae soaked in orange carbon dots when compared to the control group. In addition, fig. 3 shows how human transferrin conjugate carbon dots with a concentration of 5 mg/mL also proved to target calcified bones in 7-8dpf larvae. This is proof that promising drug delivery techniques can be formed in order to optimize and enhance treatment plans for certain lethal diseases. For instance, drug delivery methods can be used to perfect treating cancer cells while maintaining healthy cells. Using our methods and treatments, we were able to target calcified bones. This could have been due to higher concentrations of carbon dot solution and longer soaking incubation periods. Furthermore, survival rates were high and showed that soaking methods are nontoxic, benign, and successful in biological implications. Soaking with orange carbon dot solution did not exhibit targeting of calcified bone but did show targeting of organs and tissue, suggesting soaking methods can also work with various diverse types of carbon dots.

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Nov 18th, 2:15 PM Nov 18th, 3:15 PM

Promising Drug Delivery In Vivo Zebrafish Larvae Using Carbon Dots To Target Calcified Bones

BSC-Ursa Minor 121

Carbon nanoparticles, otherwise known as c-dots are a relatively new breakthrough in science with possible prospects in chemical sensing, bio imaging, and other biological applications. Due to their low toxicity levels and various top-down and bottom-up synthesis, c-dot research is high in demand. However, while carbon dots are becoming more prevalent, their generally low quantum yields, in addition to their unclear morphologies have not allowed them to outperform their metal semiconductor nanoparticle rivals. The composition of carbon dots is still widely-unknown and has called for further investigation, although they are mostly comprised of carbon, oxygen, and nitrogen. The use of injection of carbon nanoparticles on 6dpf zebrafish larvae was previously considered in addition to previous work on larvae soaking. Herein, we explored top-down and bottom-up approaches to synthesize black carbon dots, human transferrin conjugate carbon dots, and orange carbon dots. We then soaked zebrafish larvae in various concentrations to target calcified bone thus proving possible applications for drug delivery with human transferrin conjugate c-dots. After treatment, bio imaging occurred on 7dpf-9dpf where fluorescent targeted calcified bones were observed in 3.4 mg/mL, and 5 mg/mL concentrations of black carbon dots in addition to 5 mg/mL transferrin conjugate c-dots. Previous studies using soaking methods have only shown targeted organs and tissue through soaking methods with lower concentrations carbon dot solutions. Using our methods and treatments, we were able to target calcified bones. This was exhibited in soaking that occurred in 3.4, 5, and 5 mg/mL conjugated human transferrin black carbon dot solutions.