Presentation Title

Capillary Tube and Thread-Based Electrodes for the Detection of Glucose and Acetylthiocholine

Faculty Mentor

Frank A. Gomez

Start Date

23-11-2019 9:30 AM

End Date

23-11-2019 9:45 AM

Location

Markstein 203

Session

oral 1

Type of Presentation

Oral Talk

Subject Area

behavioral_social_sciences

Abstract

Rapid technology growth has greatly enhanced the scope of real-time health monitoring, facilitating the incorporation of medical diagnostics into patients’ home healthcare regimens. Reusable and simple to fabricate electrochemical sensors for the detection of glucose and acetylthiocholine (ATC) using thread-based electrodes is described. Production costs of the sensors are low since they are reusable, composed of inexpensive materials, and require minimal reagents, making them well suited for resource-limited regions throughout the world. The devices contain three thread-based electrodes (reference, working, and counter) fabricated by painting pieces of nylon thread with either layered silver ink and carbon ink or silver/silver chloride ink. The thread-based electrodes were inserted into glass capillary tubes, leaving the ends of the electrodes exposed to minimize the surface area of the exposed electrode and minimize solution volumes used for glucose detection. For the first system, glucose and ATC concentrations were measured by pipetting 12 µL of solution onto wax-printed circles on chromatography paper. The capillary tube electrodes were placed within the circles to run cyclic voltammetry (CV) for a range of glucose concentrations (0-20 mM) and ATC concentrations (0-9.84 mg/mL). Glucose concentration was plotted against the oxidation peak heights at 0.45 V of each CV (R2 = 0.966), and ATC concentration was plotted against the current output at 0.6 V (R2 = 0.984). For the second system, the electrodes were placed inside bubble wrap in which 30 µL solutions of glucose or ATC solution were spotted. After CVs were obtained, glucose (0-20 mM) concentration was plotted against current output at 0.45 V (R2 = 0.991). ATC (0-9.84 mg/mL) concentration was plotted against current output at the oxidation peak at 0.6 V (R2 = 0.982). For the third system, two nylon threads (5 inches in length) were knotted, twisted together, and pressed into 5 layers of parafilm with one thread dipped into glucose (0-15 mM) and the other dipped into a solution of GOx, PBS, and potassium ferricyanide. Through capillary action, the solutions traveled and mixed within the thread matrix, and the electrodes were placed on top of the thread to obtain CVs. Glucose concentration was plotted against current output at 0.45 V (R2 = 0.9584).

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Nov 23rd, 9:30 AM Nov 23rd, 9:45 AM

Capillary Tube and Thread-Based Electrodes for the Detection of Glucose and Acetylthiocholine

Markstein 203

Rapid technology growth has greatly enhanced the scope of real-time health monitoring, facilitating the incorporation of medical diagnostics into patients’ home healthcare regimens. Reusable and simple to fabricate electrochemical sensors for the detection of glucose and acetylthiocholine (ATC) using thread-based electrodes is described. Production costs of the sensors are low since they are reusable, composed of inexpensive materials, and require minimal reagents, making them well suited for resource-limited regions throughout the world. The devices contain three thread-based electrodes (reference, working, and counter) fabricated by painting pieces of nylon thread with either layered silver ink and carbon ink or silver/silver chloride ink. The thread-based electrodes were inserted into glass capillary tubes, leaving the ends of the electrodes exposed to minimize the surface area of the exposed electrode and minimize solution volumes used for glucose detection. For the first system, glucose and ATC concentrations were measured by pipetting 12 µL of solution onto wax-printed circles on chromatography paper. The capillary tube electrodes were placed within the circles to run cyclic voltammetry (CV) for a range of glucose concentrations (0-20 mM) and ATC concentrations (0-9.84 mg/mL). Glucose concentration was plotted against the oxidation peak heights at 0.45 V of each CV (R2 = 0.966), and ATC concentration was plotted against the current output at 0.6 V (R2 = 0.984). For the second system, the electrodes were placed inside bubble wrap in which 30 µL solutions of glucose or ATC solution were spotted. After CVs were obtained, glucose (0-20 mM) concentration was plotted against current output at 0.45 V (R2 = 0.991). ATC (0-9.84 mg/mL) concentration was plotted against current output at the oxidation peak at 0.6 V (R2 = 0.982). For the third system, two nylon threads (5 inches in length) were knotted, twisted together, and pressed into 5 layers of parafilm with one thread dipped into glucose (0-15 mM) and the other dipped into a solution of GOx, PBS, and potassium ferricyanide. Through capillary action, the solutions traveled and mixed within the thread matrix, and the electrodes were placed on top of the thread to obtain CVs. Glucose concentration was plotted against current output at 0.45 V (R2 = 0.9584).