Presentation Title

Increased growth of Human Chordoma Cells When Cultured With Human Adipose Derived Stem Cells In A Microfluidic Device

Faculty Mentor

Carlos Luna Lopez

Start Date

23-11-2019 8:45 AM

End Date

23-11-2019 9:00 AM

Location

Markstein 210

Session

oral 1

Type of Presentation

Oral Talk

Subject Area

biological_agricultural_sciences

Abstract

According to the National Institute of Health, 80 percent of adults suffer from low back pain, which correlates to degenerative disk disease. The loss of intervertebral disc elements, such as the nucleus pulposus, leads to abnormal spine mechanics, inflammatory signaling and compression of neural structures that lead to pain. Recently, undifferentiated stem cells have been used to rescue specialized cells via transfer of mitochondria and growth factors. However, it is not known how undifferentiated stem cells can aid in the regeneration of human nucleus pulposus cells. In this work, we used a microfluidic device to co-culture human adipose-derived stem cells (ADSCs) next to human chordoma UCH-1 cells. We hypothesized that UCH-1 cells will grow more when cultured next to ADSC cells. Human chordoma derived UCH-1 cells have the same genetic and morphological properties as nucleus pulposus cells and thus, serve as a model for in vitro studies. We made a microfluidic device that consisted of two wells separated by a channel, each well allows for culture of a specific cell type. When the channel is opened, it mixes the media from each cell line. The microfluidic device was fabricated using SLA resin 3D printing to create a mold. Polydimethylsiloxane (PDMS) was used to create the device which was then bonded using deep UV to a glass slide. On average we saw about 50% increase in cell number when UCH-1 cells were mixed with ADSC cells. These results indicate that even at a distance stem cells (ADSC) are able to influence the growth of UCH-1 cells. In the future, we want to check for the growth factors that affect cell proliferation, the maximum distance between cell groups at which an effect can be seen as well as the potential bidirectional effect that UCH-1 cells can have on stem cells.

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Nov 23rd, 8:45 AM Nov 23rd, 9:00 AM

Increased growth of Human Chordoma Cells When Cultured With Human Adipose Derived Stem Cells In A Microfluidic Device

Markstein 210

According to the National Institute of Health, 80 percent of adults suffer from low back pain, which correlates to degenerative disk disease. The loss of intervertebral disc elements, such as the nucleus pulposus, leads to abnormal spine mechanics, inflammatory signaling and compression of neural structures that lead to pain. Recently, undifferentiated stem cells have been used to rescue specialized cells via transfer of mitochondria and growth factors. However, it is not known how undifferentiated stem cells can aid in the regeneration of human nucleus pulposus cells. In this work, we used a microfluidic device to co-culture human adipose-derived stem cells (ADSCs) next to human chordoma UCH-1 cells. We hypothesized that UCH-1 cells will grow more when cultured next to ADSC cells. Human chordoma derived UCH-1 cells have the same genetic and morphological properties as nucleus pulposus cells and thus, serve as a model for in vitro studies. We made a microfluidic device that consisted of two wells separated by a channel, each well allows for culture of a specific cell type. When the channel is opened, it mixes the media from each cell line. The microfluidic device was fabricated using SLA resin 3D printing to create a mold. Polydimethylsiloxane (PDMS) was used to create the device which was then bonded using deep UV to a glass slide. On average we saw about 50% increase in cell number when UCH-1 cells were mixed with ADSC cells. These results indicate that even at a distance stem cells (ADSC) are able to influence the growth of UCH-1 cells. In the future, we want to check for the growth factors that affect cell proliferation, the maximum distance between cell groups at which an effect can be seen as well as the potential bidirectional effect that UCH-1 cells can have on stem cells.