Presentation Title

Comparison of Full-Length and Mature Forms of Lysyl Oxidase

Faculty Mentor

Karlo Lopez

Start Date

23-11-2019 9:30 AM

End Date

23-11-2019 9:45 AM

Location

Markstein 102

Session

oral 1

Type of Presentation

Oral Talk

Subject Area

biological_agricultural_sciences

Abstract

Lysyl oxidase (LOX) is an extracellular cuproenzyme essential in catalyzing the crosslinkages of collagen and elastin in the extracellular matrix (ECM). The enzyme's localization to the ECM, particularly in cancer cells, makes it a potential target for activated prodrugs that tend to accumulate in the tumor stroma. High LOX expression typically indicates a poor prognosis in colon, breast, prostate, and lung cancer patients. LOX is synthesized as a 50 kDa inactive proenzyme (Pro-LOX) which undergoes extracellular proteolytic processing to form a 29 kDa mature enzyme (LOX) and an 18 kDa propeptide. The LOX propeptide has been shown to inhibit the transforming activity of the H - Ras oncogene in NIH 3T3 fibroblasts; however, only the active and mature form of the LOX enzyme in the ECM is responsible for metastasis in hypoxic cells. This work aims to compare the structure and function of mature LOX and the full-length 50 kDa enzyme.

This document is currently not available here.

Share

COinS
 
Nov 23rd, 9:30 AM Nov 23rd, 9:45 AM

Comparison of Full-Length and Mature Forms of Lysyl Oxidase

Markstein 102

Lysyl oxidase (LOX) is an extracellular cuproenzyme essential in catalyzing the crosslinkages of collagen and elastin in the extracellular matrix (ECM). The enzyme's localization to the ECM, particularly in cancer cells, makes it a potential target for activated prodrugs that tend to accumulate in the tumor stroma. High LOX expression typically indicates a poor prognosis in colon, breast, prostate, and lung cancer patients. LOX is synthesized as a 50 kDa inactive proenzyme (Pro-LOX) which undergoes extracellular proteolytic processing to form a 29 kDa mature enzyme (LOX) and an 18 kDa propeptide. The LOX propeptide has been shown to inhibit the transforming activity of the H - Ras oncogene in NIH 3T3 fibroblasts; however, only the active and mature form of the LOX enzyme in the ECM is responsible for metastasis in hypoxic cells. This work aims to compare the structure and function of mature LOX and the full-length 50 kDa enzyme.