Presentation Title

Tunable rhodamine 6G ionic fluorescent nanomaterials and evaluation of chemotherapeutic properties using stem cells

Faculty Mentor

Dr. David Bwambok

Start Date

23-11-2019 11:00 AM

End Date

23-11-2019 11:15 AM

Location

Markstein 102

Session

oral 2

Type of Presentation

Oral Talk

Subject Area

physical_mathematical_sciences

Abstract

Studies have found that rhodamine 6G (rhod 6G) cationic dyes exhibit selective toxicity towards cancer cells to which they have high affinity towards the mitochondria due to its negatively charged membrane. Our group was focused on rhod 6G cation-based nanomaterials paired with various anions. Various nanomaterials were synthesized and their specificity for localizing in the mitochondria in adipose-derived stem cells and human embryonic kidney (HEK 293) cancer cells was evaluated through fluorescent cell imaging. The rhod 6G-based nanomaterials were prepared in buffer and cell media via an additive-free reprecipitation method. These nanomaterials showed fluorescent stability and tunable chemotherapeutic properties. Fluorescence of the rhod 6G-based nanoparticles was monitored over a span of 25 days with about 99% retention of fluorescent emission for all nanoparticles. Fluorescence microscopy revealed that the rhod 6G-based nanoparticles localized in the stem cells and embryonic kidney cancer cells. These studies suggest that there is potential to enhance the selective toxicity toward cancer cells in order to develop a more effective cancer therapy by creating nanoparticles that are nontoxic to normal cells but toxic to cancer cells.

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Nov 23rd, 11:00 AM Nov 23rd, 11:15 AM

Tunable rhodamine 6G ionic fluorescent nanomaterials and evaluation of chemotherapeutic properties using stem cells

Markstein 102

Studies have found that rhodamine 6G (rhod 6G) cationic dyes exhibit selective toxicity towards cancer cells to which they have high affinity towards the mitochondria due to its negatively charged membrane. Our group was focused on rhod 6G cation-based nanomaterials paired with various anions. Various nanomaterials were synthesized and their specificity for localizing in the mitochondria in adipose-derived stem cells and human embryonic kidney (HEK 293) cancer cells was evaluated through fluorescent cell imaging. The rhod 6G-based nanomaterials were prepared in buffer and cell media via an additive-free reprecipitation method. These nanomaterials showed fluorescent stability and tunable chemotherapeutic properties. Fluorescence of the rhod 6G-based nanoparticles was monitored over a span of 25 days with about 99% retention of fluorescent emission for all nanoparticles. Fluorescence microscopy revealed that the rhod 6G-based nanoparticles localized in the stem cells and embryonic kidney cancer cells. These studies suggest that there is potential to enhance the selective toxicity toward cancer cells in order to develop a more effective cancer therapy by creating nanoparticles that are nontoxic to normal cells but toxic to cancer cells.