Presentation Title

Evaluation of delivery of near infrared fluorescent ionic nanoparticles into tumors using stem cells

Faculty Mentor

David Bwambok, Carlos Luna Lopez

Start Date

23-11-2019 8:00 AM

End Date

23-11-2019 8:45 AM

Location

207

Session

poster 1

Type of Presentation

Poster

Subject Area

physical_mathematical_sciences

Abstract

Near infrared dyes have gained increased attention for cell and tissue imaging due to low absorptivity of tissues resulting in in improved signal when NIR dyes are used as contrast agents. This study focused on NIR dyes cation (IR 786 and HMT) paired with various anions. By varying the anion, dyes with different emission properties were obtained. Additionally, the variation in the anion showed different cellular uptake properties. For example HMT BETI localized in the mitochondria. Various nanomaterials were synthesized using reprecipitation and their specificity for localizing in the mitochondria in adipose- derived stem cells and human embryonic kidney (HEK 293) cancer cells was evaluated through fluorescent cell imaging. The NIR ionic nanomaterials were prepared in buffer and cell media and these nanomaterials showed cellular uptake and localization in the mitochondria a well as selective toxicity to tumor cells and nontoxic to stem cells. These studies suggest that there is potential to enhance the selective toxicity toward cancer cells in order to develop a more effective cancer therapy by creating nanoparticles that are nontoxic to normal cells but toxic to cancer cells.

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Nov 23rd, 8:00 AM Nov 23rd, 8:45 AM

Evaluation of delivery of near infrared fluorescent ionic nanoparticles into tumors using stem cells

207

Near infrared dyes have gained increased attention for cell and tissue imaging due to low absorptivity of tissues resulting in in improved signal when NIR dyes are used as contrast agents. This study focused on NIR dyes cation (IR 786 and HMT) paired with various anions. By varying the anion, dyes with different emission properties were obtained. Additionally, the variation in the anion showed different cellular uptake properties. For example HMT BETI localized in the mitochondria. Various nanomaterials were synthesized using reprecipitation and their specificity for localizing in the mitochondria in adipose- derived stem cells and human embryonic kidney (HEK 293) cancer cells was evaluated through fluorescent cell imaging. The NIR ionic nanomaterials were prepared in buffer and cell media and these nanomaterials showed cellular uptake and localization in the mitochondria a well as selective toxicity to tumor cells and nontoxic to stem cells. These studies suggest that there is potential to enhance the selective toxicity toward cancer cells in order to develop a more effective cancer therapy by creating nanoparticles that are nontoxic to normal cells but toxic to cancer cells.