Presentation Title

Comparison of GC-MS, LC-MS, LC-MS/MS in Qualitative and Quantitative Analyses

Faculty Mentor

Jacqueline A. Trischman

Start Date

23-11-2019 8:00 AM

End Date

23-11-2019 8:45 AM

Location

227

Session

poster 1

Type of Presentation

Poster

Subject Area

physical_mathematical_sciences

Abstract

Liquid chromatography and mass spectrometry (LC-MS) is a powerful instrument in chemistry that combines separation and analytical capabilities. It can be applied to analyze a wide range of organic compounds. It is important to understand the characteristics and benefits of different types of mass analyses, so one can choose the method best suited for the needs of the experiments. To show this, we analyzed several molecules with the same nominal molecular weight of 152 amu using GC-MS and LC-MS single quad mass analysis. By adding the LC-MS/MS features, we could distinguish between the molecules. The results of using GC-MS, LC-MS, LC-MS/MS will allow us to demonstrate that LC-MS/MS offers better sensitivity and selectivity than GC-MS or LC-MS with single quadrupole analysis. Results will be shown for pulegone, carveol, fenchone, vanillin, and methyl salicylate. This work will be used to developed a new instrumental undergraduate experiment and to instruct independent research students in the future.

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Nov 23rd, 8:00 AM Nov 23rd, 8:45 AM

Comparison of GC-MS, LC-MS, LC-MS/MS in Qualitative and Quantitative Analyses

227

Liquid chromatography and mass spectrometry (LC-MS) is a powerful instrument in chemistry that combines separation and analytical capabilities. It can be applied to analyze a wide range of organic compounds. It is important to understand the characteristics and benefits of different types of mass analyses, so one can choose the method best suited for the needs of the experiments. To show this, we analyzed several molecules with the same nominal molecular weight of 152 amu using GC-MS and LC-MS single quad mass analysis. By adding the LC-MS/MS features, we could distinguish between the molecules. The results of using GC-MS, LC-MS, LC-MS/MS will allow us to demonstrate that LC-MS/MS offers better sensitivity and selectivity than GC-MS or LC-MS with single quadrupole analysis. Results will be shown for pulegone, carveol, fenchone, vanillin, and methyl salicylate. This work will be used to developed a new instrumental undergraduate experiment and to instruct independent research students in the future.