Presentation Title

Exploring the Dynamics of Biological Macromolecules at Angstrom Scale

Faculty Mentor

Zahra Alavi

Start Date

23-11-2019 8:00 AM

End Date

23-11-2019 8:45 AM

Location

241

Session

poster 1

Type of Presentation

Poster

Subject Area

physical_mathematical_sciences

Abstract

This research project studied Guanylate Kinase (GK) using nano-rheological techniques. These biological macromolecules were immersed in solutions with different solvents including dimethyl sulfoxide (DMSO) in order to observe how the conformational dynamics of the molecules change.

An oscillatory force was applied by attaching enzymes to a gold-coated surface and gold nanoparticles. An additional gold-coated surface was placed on top of the setup in order to create a parallel plate capacitor configuration. An oscillatory voltage was then applied across the capacitor to drive the gold nanoparticles and exert a force on the enzymes. The setup detected the ensemble averaged movement of the enzymes at the Angstrom scale in order to measure the conformational dynamics.

The specific goals of this research project included the following: create a one-of-a-kind experimental setup which will be useful for a plethora of other experiments in the future, deform enzymes and record their levels of deformation, change what enzymes and solvents are used, and compare data from the experiments.

The experimental setup took the most time to prepare, but the procedure can now be executed with GK enzyme and various solvents. The results of the enzyme deformation revealed that they conformationally change according to a viscoelastic regime. At low frequency oscillations, the enzymes acted like a viscous fluid while at high frequencies, the enzymes were elastic like a stiff spring. Adding DMSO lowered the viscoelasticity, so the next steps for the research include changing the enzyme under study and testing more solvents. These results will be useful for further research in the fields of Biophysics, Biology, and Chemistry.

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Nov 23rd, 8:00 AM Nov 23rd, 8:45 AM

Exploring the Dynamics of Biological Macromolecules at Angstrom Scale

241

This research project studied Guanylate Kinase (GK) using nano-rheological techniques. These biological macromolecules were immersed in solutions with different solvents including dimethyl sulfoxide (DMSO) in order to observe how the conformational dynamics of the molecules change.

An oscillatory force was applied by attaching enzymes to a gold-coated surface and gold nanoparticles. An additional gold-coated surface was placed on top of the setup in order to create a parallel plate capacitor configuration. An oscillatory voltage was then applied across the capacitor to drive the gold nanoparticles and exert a force on the enzymes. The setup detected the ensemble averaged movement of the enzymes at the Angstrom scale in order to measure the conformational dynamics.

The specific goals of this research project included the following: create a one-of-a-kind experimental setup which will be useful for a plethora of other experiments in the future, deform enzymes and record their levels of deformation, change what enzymes and solvents are used, and compare data from the experiments.

The experimental setup took the most time to prepare, but the procedure can now be executed with GK enzyme and various solvents. The results of the enzyme deformation revealed that they conformationally change according to a viscoelastic regime. At low frequency oscillations, the enzymes acted like a viscous fluid while at high frequencies, the enzymes were elastic like a stiff spring. Adding DMSO lowered the viscoelasticity, so the next steps for the research include changing the enzyme under study and testing more solvents. These results will be useful for further research in the fields of Biophysics, Biology, and Chemistry.