Presentation Title

Effects of Lysergic Acid Diethylamide in Representation-Mediated Learning in Rat Model

Faculty Mentor

Jane Taylor

Start Date

23-11-2019 8:00 AM

End Date

23-11-2019 8:45 AM

Location

25

Session

poster 1

Type of Presentation

Poster

Subject Area

behavioral_social_sciences

Abstract

Hallucinations are perceptual experiences of stimuli that are not externally present. While hallucinations are a common symptom among psychotic disorders, they are also experienced among the general population and can be elicited by drugs like lysergic acid diethylamide (LSD), a serotoninergic 2A-receptor agonist. In a controlled setting, sensory conditioning has also been shown to induce hallucinations, particularly in people prone to hallucinations, where an absent stimulus is perceived simply by presenting a cue previously paired with it. In animals, the ability to bring up strong, sensory representations of cues through presentation of a paired stimulus has been used to model this phenomenon. Here, we used a representation-mediated taste aversion behavioral paradigm (RMTA) where rats received flavor (sucrose/maltodextrin) and odor (banana/almond) pairings, then one odor was devalued with an injection of lithium chloride, and we later tested whether its associated flavor was also devalued. We optimized this protocol such that rats show strong aversion to the odors directly paired with nausea, but do notshow an aversion to the paired flavor. Forty-two male Sprague-Dawley rats underwent RMTA and were administered with either LSD or saline on the odor-LiCl training days. We then determined whether LSD increases the extent to which the representation of the flavor is elicited and therefore devalued. A three-way mixed analysis of variance revealed a significant main effect for flavor type (F=13.084, p=.0006). Regardless of the presence of lithium chloride, sucrose was overall preferred (sucrose-preference).Additionally, a significant interaction was shown between the lithium chloride pairing condition (Control, LSD Aversive, LSD Neutral) and flavor (F=3.962, p

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Nov 23rd, 8:00 AM Nov 23rd, 8:45 AM

Effects of Lysergic Acid Diethylamide in Representation-Mediated Learning in Rat Model

25

Hallucinations are perceptual experiences of stimuli that are not externally present. While hallucinations are a common symptom among psychotic disorders, they are also experienced among the general population and can be elicited by drugs like lysergic acid diethylamide (LSD), a serotoninergic 2A-receptor agonist. In a controlled setting, sensory conditioning has also been shown to induce hallucinations, particularly in people prone to hallucinations, where an absent stimulus is perceived simply by presenting a cue previously paired with it. In animals, the ability to bring up strong, sensory representations of cues through presentation of a paired stimulus has been used to model this phenomenon. Here, we used a representation-mediated taste aversion behavioral paradigm (RMTA) where rats received flavor (sucrose/maltodextrin) and odor (banana/almond) pairings, then one odor was devalued with an injection of lithium chloride, and we later tested whether its associated flavor was also devalued. We optimized this protocol such that rats show strong aversion to the odors directly paired with nausea, but do notshow an aversion to the paired flavor. Forty-two male Sprague-Dawley rats underwent RMTA and were administered with either LSD or saline on the odor-LiCl training days. We then determined whether LSD increases the extent to which the representation of the flavor is elicited and therefore devalued. A three-way mixed analysis of variance revealed a significant main effect for flavor type (F=13.084, p=.0006). Regardless of the presence of lithium chloride, sucrose was overall preferred (sucrose-preference).Additionally, a significant interaction was shown between the lithium chloride pairing condition (Control, LSD Aversive, LSD Neutral) and flavor (F=3.962, p