Presentation Title

The Contribution of Hops Oil Extracts on Colon Cancer Cell Growth

Faculty Mentor

Dr. Sarah Forester

Start Date

23-11-2019 8:00 AM

End Date

23-11-2019 8:45 AM

Location

101

Session

poster 1

Type of Presentation

Poster

Subject Area

biological_agricultural_sciences

Abstract

Terpenes, phenolics, alpha-acids, and beta-acids derived from hops (Humulus lupulus) contribute to the flavour, and preservative characteristics of craft beer. This study investigates the contribution of these intrinsic bioactive components from hops in the potential anticancer activity of beer. Two beers were chosen as precedence for hops selection, El Cerrito a Mexican lager with a 22IBU index, and Hollow Body an Indian Pale Ale with a 44IBU index. From Hollow body, the hops used were Citra & El Dorado hops; and from El Cerrito, Saaz hops. To extract the essential oils an ethanol soxhlet extraction was carried out to extract the alpha- and beta-acids, winterized to precipitate the lipids, and distilled to get rid of any remaining ethanol. The two oil extracts, Citra/El Dorado and Saaz, were dissolved in dimethyl sulfoxide (DMSO) before being resuspended in cell culture media at varying diluted concentrations. HT-29 and Caco-2 human colon cancer cells were incubated with various concentrations (0-1000 ppm) of the oil extract treatments for 24 hours. DMSO was added to all treated and control cells, 0.1%(v/v). Following incubation, cell viability was quantified via thiazolyl blue tetrazolium bromide(MTT) assay. The Saaz and Citra/El Dorado oil extracts inhibited the growth of the HT-29 cells in a dose-dependent manner. At a concentration of 500ppm, the Saaz and Citra/El Dorado oil extracts inhibited cell viability by 88.6% and 83.1%. The Saaz and Citra/El Dorado oil extracts inhibited cell viability of Caco-2 cells by 91.8% and 90.0%. This level of inhibition was comparable to a previous experiment in our lab where HT-29 cells were treated with aqueous hops extracts. We conclude that hops may contribute to the potential anticancer effects of beer, whether it is made using ethanol-based oil extracts or water-based freeze-dried extracts.

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Nov 23rd, 8:00 AM Nov 23rd, 8:45 AM

The Contribution of Hops Oil Extracts on Colon Cancer Cell Growth

101

Terpenes, phenolics, alpha-acids, and beta-acids derived from hops (Humulus lupulus) contribute to the flavour, and preservative characteristics of craft beer. This study investigates the contribution of these intrinsic bioactive components from hops in the potential anticancer activity of beer. Two beers were chosen as precedence for hops selection, El Cerrito a Mexican lager with a 22IBU index, and Hollow Body an Indian Pale Ale with a 44IBU index. From Hollow body, the hops used were Citra & El Dorado hops; and from El Cerrito, Saaz hops. To extract the essential oils an ethanol soxhlet extraction was carried out to extract the alpha- and beta-acids, winterized to precipitate the lipids, and distilled to get rid of any remaining ethanol. The two oil extracts, Citra/El Dorado and Saaz, were dissolved in dimethyl sulfoxide (DMSO) before being resuspended in cell culture media at varying diluted concentrations. HT-29 and Caco-2 human colon cancer cells were incubated with various concentrations (0-1000 ppm) of the oil extract treatments for 24 hours. DMSO was added to all treated and control cells, 0.1%(v/v). Following incubation, cell viability was quantified via thiazolyl blue tetrazolium bromide(MTT) assay. The Saaz and Citra/El Dorado oil extracts inhibited the growth of the HT-29 cells in a dose-dependent manner. At a concentration of 500ppm, the Saaz and Citra/El Dorado oil extracts inhibited cell viability by 88.6% and 83.1%. The Saaz and Citra/El Dorado oil extracts inhibited cell viability of Caco-2 cells by 91.8% and 90.0%. This level of inhibition was comparable to a previous experiment in our lab where HT-29 cells were treated with aqueous hops extracts. We conclude that hops may contribute to the potential anticancer effects of beer, whether it is made using ethanol-based oil extracts or water-based freeze-dried extracts.