Presentation Title

Effect of total flavonoid extracts of chamomile (Chamomilla recutita) and elecampane (Inula helenium) on volume regulation and the volume-sensitive anion channel

Faculty Mentor

Ravshan Z. Sabirov

Start Date

23-11-2019 8:00 AM

End Date

23-11-2019 8:45 AM

Location

107

Session

poster 1

Type of Presentation

Poster

Subject Area

biological_agricultural_sciences

Abstract

The volume-sensitive outward rectifying (VSOR) channel plays a major role in cell volume regulation of organisms and is a vital component needed for survival. The purpose of the present study was to determine the effect that flavonoids present in chamomile and elecampane have on VSOR channel. The experiments were carried out on an EPC-9 patch-clamp system and photometer MKMF-01. The EPC-9 patch-clamp is an advantageous versatile set up that allows for intracellular voltage as well as cell membrane current and capacitance to be measured quantitatively and in real time. The results showed that the current produced by the swelling from activated VSOR was irreversibly and completely abolished by the chamomile and elecampane extracts on the U937 cell. The U937 cells are originated from the myeloid lineage and were isolated from an aggressive type of non-Hodgkin lymphoma. They are commonly used in biomedical research for the study of monocyte differentiation and is the reason they were used in this study. The plant extracts from chamomile and elecampane both demonstrated thymocyte cell volume regulation inhibition with a biphasic nature at an overall half-maximal dose of 14.4 ± 7.7 μg/mL and 34.3 ± 8.4 μg/mL, respectively. Flavonoids represent a natural abundant subset of polyphenols which can potentially be used for the suppression of cell inhibition of VSOR channel without the harmful consequences of synthetic drugs.

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Nov 23rd, 8:00 AM Nov 23rd, 8:45 AM

Effect of total flavonoid extracts of chamomile (Chamomilla recutita) and elecampane (Inula helenium) on volume regulation and the volume-sensitive anion channel

107

The volume-sensitive outward rectifying (VSOR) channel plays a major role in cell volume regulation of organisms and is a vital component needed for survival. The purpose of the present study was to determine the effect that flavonoids present in chamomile and elecampane have on VSOR channel. The experiments were carried out on an EPC-9 patch-clamp system and photometer MKMF-01. The EPC-9 patch-clamp is an advantageous versatile set up that allows for intracellular voltage as well as cell membrane current and capacitance to be measured quantitatively and in real time. The results showed that the current produced by the swelling from activated VSOR was irreversibly and completely abolished by the chamomile and elecampane extracts on the U937 cell. The U937 cells are originated from the myeloid lineage and were isolated from an aggressive type of non-Hodgkin lymphoma. They are commonly used in biomedical research for the study of monocyte differentiation and is the reason they were used in this study. The plant extracts from chamomile and elecampane both demonstrated thymocyte cell volume regulation inhibition with a biphasic nature at an overall half-maximal dose of 14.4 ± 7.7 μg/mL and 34.3 ± 8.4 μg/mL, respectively. Flavonoids represent a natural abundant subset of polyphenols which can potentially be used for the suppression of cell inhibition of VSOR channel without the harmful consequences of synthetic drugs.