Presentation Title

Investigating the Effects of UV Radiation on PRMT1 Methylation of Histone H4

Presenter Information

Alejandra LealFollow

Faculty Mentor

Dr. Cecilia Zurita-Lopez

Start Date

23-11-2019 8:00 AM

End Date

23-11-2019 8:45 AM

Location

109

Session

poster 1

Type of Presentation

Poster

Subject Area

biological_agricultural_sciences

Abstract

Abstract

UV irradiation can lead to cancer by directly damaging DNA or by altering the activity of enzymes that modify histone proteins, which contain amino-terminal tails that become post-translationally modified, leading to changes in gene expression. This project investigates the effect of UV light (280-315nm) on the formation of asymmetric dimethylation at arginine residue 3 in histone H4 (H4R3adma) catalyzed by the enzyme PRMT1. We hypothesize that when exposed to UV light, the enzymatic activity of PRMT1 will increase causing greater formation of H4R3adma. Methylation reactions were carried out in vitro using recombinant bacterially expressed GST-PRMT1 constructs and commercially purchased full-length histone H4 protein. The reactions were carried out in the presence or absence of UVB light at 37°C for 1 hour. While the effects of UVB light on H4R3adma by PRMT1 have yet to be validated, preliminary evidence suggests that UVB exposure causes an increase in automethylation of PRMT1 as well as an increase in H4R3adma product. In addition, the development of this assay using histone H4 extracted from mammalian cells exposed to UVB is also underway. A greater understanding of transcriptional regulation and UVB damage response pathways will be gained by investigating UV irradiation on H4R3adma by PRMT1.

Keywords

Post-translational modification, UV radiation, Methylation, Histone, gene expression

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Nov 23rd, 8:00 AM Nov 23rd, 8:45 AM

Investigating the Effects of UV Radiation on PRMT1 Methylation of Histone H4

109

Abstract

UV irradiation can lead to cancer by directly damaging DNA or by altering the activity of enzymes that modify histone proteins, which contain amino-terminal tails that become post-translationally modified, leading to changes in gene expression. This project investigates the effect of UV light (280-315nm) on the formation of asymmetric dimethylation at arginine residue 3 in histone H4 (H4R3adma) catalyzed by the enzyme PRMT1. We hypothesize that when exposed to UV light, the enzymatic activity of PRMT1 will increase causing greater formation of H4R3adma. Methylation reactions were carried out in vitro using recombinant bacterially expressed GST-PRMT1 constructs and commercially purchased full-length histone H4 protein. The reactions were carried out in the presence or absence of UVB light at 37°C for 1 hour. While the effects of UVB light on H4R3adma by PRMT1 have yet to be validated, preliminary evidence suggests that UVB exposure causes an increase in automethylation of PRMT1 as well as an increase in H4R3adma product. In addition, the development of this assay using histone H4 extracted from mammalian cells exposed to UVB is also underway. A greater understanding of transcriptional regulation and UVB damage response pathways will be gained by investigating UV irradiation on H4R3adma by PRMT1.

Keywords

Post-translational modification, UV radiation, Methylation, Histone, gene expression