Presentation Title

Effects of Hypocretin 2 Receptor Antagonist on Fragmentation of Maternal Care

Faculty Mentor

Kimberly D'Anna-Hernandez

Start Date

23-11-2019 8:00 AM

End Date

23-11-2019 8:45 AM

Location

15

Session

poster 1

Type of Presentation

Poster

Subject Area

behavioral_social_sciences

Abstract

Postpartum depression is a common disorder that disrupts maternal care and can have detrimental effects on offspring. In mice, fragmented interactions between the dam and her pups have been shown to disrupt the maternal sensory signals pups need to develop properly . Hypocretin (HCRT), a neuropeptide that is upregulated during lactation, is linked to motivation and arousal which are important processes of maternal care. The HCRT system is composed of two receptors with differential behavioral functions. HCRT receptor 1 (HCRTR1) is associated with reward whereas HCRT receptor 2 (HCRTR2) is linked to arousal. Intermediate doses of a HCRTR1 antagonist have been found to increase rewarding maternal behaviors, but the role of HCRTR2 in maternal care is unknown. We hypothesized that the dams that received a high dose of the HCRTR2 antagonist would show more depressive-like behaviors, as measured by fragmented behavior, than those who received a low dose or the vehicle. On postnatal day 4, each dam(n=32) received an i.p. injection of either a HCRTR2 antagonist (n=11, 50kg/kg; n=11, 25 mg/kg) or a vehicle. Dams then underwent a Tail-suspension test before being placed in their home cage for a maternal behavior observation. This maternal behavior was analyzed for fragmentation by examining how often animals switched between maternal and non-maternal behaviors. A oneway ANOVA revealed a significant effect of HCRTR2 antagonist on dams’ fragmented behavior, F(2,30)=8.958, p=.001. A Tukey’s post hoc analysis revealed that dams who received both the low dose (M = .059, SEM = .031) and high dose (M = .094, SEM = .034) showed less fragmented behavior than the dams that received the vehicle(M = .267, SEM = .043). Results suggest that blocking HCRTR2 promotes consistent and predictable maternal care. Thus, HCRT may be an important neuromechanism in the regulation of depressive-like behaviors in the postpartum period.

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Nov 23rd, 8:00 AM Nov 23rd, 8:45 AM

Effects of Hypocretin 2 Receptor Antagonist on Fragmentation of Maternal Care

15

Postpartum depression is a common disorder that disrupts maternal care and can have detrimental effects on offspring. In mice, fragmented interactions between the dam and her pups have been shown to disrupt the maternal sensory signals pups need to develop properly . Hypocretin (HCRT), a neuropeptide that is upregulated during lactation, is linked to motivation and arousal which are important processes of maternal care. The HCRT system is composed of two receptors with differential behavioral functions. HCRT receptor 1 (HCRTR1) is associated with reward whereas HCRT receptor 2 (HCRTR2) is linked to arousal. Intermediate doses of a HCRTR1 antagonist have been found to increase rewarding maternal behaviors, but the role of HCRTR2 in maternal care is unknown. We hypothesized that the dams that received a high dose of the HCRTR2 antagonist would show more depressive-like behaviors, as measured by fragmented behavior, than those who received a low dose or the vehicle. On postnatal day 4, each dam(n=32) received an i.p. injection of either a HCRTR2 antagonist (n=11, 50kg/kg; n=11, 25 mg/kg) or a vehicle. Dams then underwent a Tail-suspension test before being placed in their home cage for a maternal behavior observation. This maternal behavior was analyzed for fragmentation by examining how often animals switched between maternal and non-maternal behaviors. A oneway ANOVA revealed a significant effect of HCRTR2 antagonist on dams’ fragmented behavior, F(2,30)=8.958, p=.001. A Tukey’s post hoc analysis revealed that dams who received both the low dose (M = .059, SEM = .031) and high dose (M = .094, SEM = .034) showed less fragmented behavior than the dams that received the vehicle(M = .267, SEM = .043). Results suggest that blocking HCRTR2 promotes consistent and predictable maternal care. Thus, HCRT may be an important neuromechanism in the regulation of depressive-like behaviors in the postpartum period.