Presentation Title

Investigating Salivary and Serum Albumin Measurement for Non-Clinical Settings

Faculty Mentor

Dr.Ertan Salik , Dr.Erkan Ozkaya

Start Date

23-11-2019 8:00 AM

End Date

23-11-2019 8:45 AM

Location

185

Session

poster 1

Type of Presentation

Poster

Subject Area

health_nutrition_clinical_science

Abstract

We developed a simple system that enables one to measure salivary and serum albumin levels in non-clinical settings. Total protein measurement is a common clinical test which measures the total concentration of albumin and globulin, as well as the ratio of these two protein groups present in the blood serum of a patient. This test is performed to ascertain whether the patient tested has nutritional deficiencies or damage to the kidney or liver. Abnormal concentration levels of protein in serum can indicate chronic inflammation or infection in above normal concentrations and diseases like malnutrition and liver disease in below normal concentrations. A widely used method for these tests utilizes the Biuret reagent, which is an assay with a colorimetric reaction proportional to the concentration of protein in solution. In a series of experiments, we utilized the Biuret reagent to measure Bovine Serum Albumin (BSA) in Phosphate-Buffered Saline (PBS) solution at concentration ranges expected in blood serum and saliva, then captured images of the results using a digital camera and analyzed the images using the NIH image processing program, ImageJ. From our series of experiments, we concluded that our analysis method was largely successful in determining concentration accuracy with predicted measurements to actual values. Specifically, the success rate for our final trial resulted in ~77% accuracy of our predicted values to our actual values. We will present our calibration curves, predicted concentration values, and error analysis that were used to determine the success of the method.

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Nov 23rd, 8:00 AM Nov 23rd, 8:45 AM

Investigating Salivary and Serum Albumin Measurement for Non-Clinical Settings

185

We developed a simple system that enables one to measure salivary and serum albumin levels in non-clinical settings. Total protein measurement is a common clinical test which measures the total concentration of albumin and globulin, as well as the ratio of these two protein groups present in the blood serum of a patient. This test is performed to ascertain whether the patient tested has nutritional deficiencies or damage to the kidney or liver. Abnormal concentration levels of protein in serum can indicate chronic inflammation or infection in above normal concentrations and diseases like malnutrition and liver disease in below normal concentrations. A widely used method for these tests utilizes the Biuret reagent, which is an assay with a colorimetric reaction proportional to the concentration of protein in solution. In a series of experiments, we utilized the Biuret reagent to measure Bovine Serum Albumin (BSA) in Phosphate-Buffered Saline (PBS) solution at concentration ranges expected in blood serum and saliva, then captured images of the results using a digital camera and analyzed the images using the NIH image processing program, ImageJ. From our series of experiments, we concluded that our analysis method was largely successful in determining concentration accuracy with predicted measurements to actual values. Specifically, the success rate for our final trial resulted in ~77% accuracy of our predicted values to our actual values. We will present our calibration curves, predicted concentration values, and error analysis that were used to determine the success of the method.