Presentation Title

Femtosecond Infrared Laser Spectroscopy: An Approach to the Characterization of Bioconjugated Gold Nanoparticles

Faculty Mentor

Jared M Ashcroft, Aziz Boulesbaa

Start Date

23-11-2019 8:45 AM

End Date

23-11-2019 9:30 AM

Location

208

Session

poster 2

Type of Presentation

Poster

Subject Area

interdisciplinary

Abstract

In past medical advancements, gold nanoparticles (AuNP) have been found useful for the diagnosis and drug delivery treatment of a multitude of illnesses. Covalently bonded peptides and antibodies have shown great promise. By attaching targeting molecules such as antibodies to AuNP, not only does it have the potential to treat illnesses like cancer, it can also be developed into a powerful aid in identifying disease or illness at an early stage.

Conjugation protocols have been optimized for functionalized gold carboxyl (-COOH) to protein A amine (-NH3) linkage. The reaction between negatively charged carboxylic acid gold nanoparticles and positively charged primary amines such as protein A-NH3 yields to covalent conjugation. However, characterization of AuNP bioconjugates is imperative for reproducibility and the efficiency of its performance. Some of the important characterizations to be made of AuNP bioconjugates would be to identify structure and biomolecular orientation, surface characteristics, AuNP-bioconjugate stability, the ratio of nanomaterial to biomolecule, solubility, and concentration.

This research seeks to employ optical techniques to examine the ultrafast phenomena that occur at the interface of nanomaterials and biomolecules. In order to obtain a clean spectral region, prior to probing the bioconjugate sample, a phase transfer from aqueous conditions to an organic solvent is required. Subsequently, the interfaces can then be characterized on a femtosecond time scale by studying the vibrational energy of the biomolecule and localized surface plasmon resonance (LSPR) of AuNP using infrared spectroscopy. A femtosecond white-light continuum is used to probe the dynamics of LSPRs, and a mid-infrared (mid-IR) probe is used to probe the vibrational dynamics in the biomolecule. These dynamics will be correlated in order to make conclusions regarding the energy transfer at the interface.

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Nov 23rd, 8:45 AM Nov 23rd, 9:30 AM

Femtosecond Infrared Laser Spectroscopy: An Approach to the Characterization of Bioconjugated Gold Nanoparticles

208

In past medical advancements, gold nanoparticles (AuNP) have been found useful for the diagnosis and drug delivery treatment of a multitude of illnesses. Covalently bonded peptides and antibodies have shown great promise. By attaching targeting molecules such as antibodies to AuNP, not only does it have the potential to treat illnesses like cancer, it can also be developed into a powerful aid in identifying disease or illness at an early stage.

Conjugation protocols have been optimized for functionalized gold carboxyl (-COOH) to protein A amine (-NH3) linkage. The reaction between negatively charged carboxylic acid gold nanoparticles and positively charged primary amines such as protein A-NH3 yields to covalent conjugation. However, characterization of AuNP bioconjugates is imperative for reproducibility and the efficiency of its performance. Some of the important characterizations to be made of AuNP bioconjugates would be to identify structure and biomolecular orientation, surface characteristics, AuNP-bioconjugate stability, the ratio of nanomaterial to biomolecule, solubility, and concentration.

This research seeks to employ optical techniques to examine the ultrafast phenomena that occur at the interface of nanomaterials and biomolecules. In order to obtain a clean spectral region, prior to probing the bioconjugate sample, a phase transfer from aqueous conditions to an organic solvent is required. Subsequently, the interfaces can then be characterized on a femtosecond time scale by studying the vibrational energy of the biomolecule and localized surface plasmon resonance (LSPR) of AuNP using infrared spectroscopy. A femtosecond white-light continuum is used to probe the dynamics of LSPRs, and a mid-infrared (mid-IR) probe is used to probe the vibrational dynamics in the biomolecule. These dynamics will be correlated in order to make conclusions regarding the energy transfer at the interface.