Presentation Title

Examining a Functional Interaction Between Chd1 and Histone H1 in Drosophila Melanogaster

Presenter Information

Breanna KimFollow

Faculty Mentor

Dr. Jennifer Armstrong

Start Date

23-11-2019 8:45 AM

End Date

23-11-2019 9:30 AM

Location

254

Session

poster 2

Type of Presentation

Poster

Subject Area

physical_mathematical_sciences

Abstract

Over-expression of the highly conserved chromatin remodeling factor Chd1 (Chromodomain-helicase-DNA-binding protein 1), has been implicated to act in opposition to other remodeling factors, and has been associated with a knockdown of Histone H1 function. H1 (previously Linker Histone H1) is critical to the maintenance of nucleosome structure. Chd1 is a commonly deleted gene in human prostate cancer, and its loss is associated with aggressive disease. In Drosophila melanogaster, Chd1 overexpression is associated severe, fully penetrant wing defect phenotypes. This study aims to clarify the interaction between Chd1 and Histone H1 by making use of a genetic assay in Drosophila melanogaster by further knocking down H1 function. The genetic assay relies on the severity of wing defects to score progeny and demonstrate possible functional interactions. Our preliminary results suggest that there is a functional interaction between Chd1 and H1. Ongoing research clarifies the exact nature of these interactions.

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Nov 23rd, 8:45 AM Nov 23rd, 9:30 AM

Examining a Functional Interaction Between Chd1 and Histone H1 in Drosophila Melanogaster

254

Over-expression of the highly conserved chromatin remodeling factor Chd1 (Chromodomain-helicase-DNA-binding protein 1), has been implicated to act in opposition to other remodeling factors, and has been associated with a knockdown of Histone H1 function. H1 (previously Linker Histone H1) is critical to the maintenance of nucleosome structure. Chd1 is a commonly deleted gene in human prostate cancer, and its loss is associated with aggressive disease. In Drosophila melanogaster, Chd1 overexpression is associated severe, fully penetrant wing defect phenotypes. This study aims to clarify the interaction between Chd1 and Histone H1 by making use of a genetic assay in Drosophila melanogaster by further knocking down H1 function. The genetic assay relies on the severity of wing defects to score progeny and demonstrate possible functional interactions. Our preliminary results suggest that there is a functional interaction between Chd1 and H1. Ongoing research clarifies the exact nature of these interactions.