Presentation Title

Gene Expression Profiling Across Life History Stages of Hippodemia convergens

Faculty Mentor

Arun Sethuraman

Start Date

23-11-2019 8:45 AM

End Date

23-11-2019 9:30 AM

Location

76

Session

poster 2

Type of Presentation

Poster

Subject Area

biological_agricultural_sciences

Abstract

Hippodemia convergens, also called the convergent lady beetle is used extensively in biological control of aphids, whiteflies, and scale insects. Unlike most lady beetles, the convergent beetle displays aggregation behaviors during the winter. Beetles are commonly collected in this area and distributed for biological control. We hypothesize that transcriptome data could lead to a better understanding of the genes involved in their convergent behaviors. We preformed whole RNA extractions from flash frozen homogenized tissue of instars, pupae, and adults of an Eastern (Kansas City, MO) population of Hippodamia convergens for further analysis of gene expression profiling across life history stages. Whole RNA was then subjected to NGS library preparation. Only two of the samples yielded quality libraries for sequencing. Our future research plans are to send the prepared libraries for sequencing and compile and annotate the transcriptome of H. convergens. Transcriptomic data could potentially be used to isolate compounds associated with H. convergens aggregation behavior and be of use within the biological control industry to attract lady beetles as natural enemies.

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Nov 23rd, 8:45 AM Nov 23rd, 9:30 AM

Gene Expression Profiling Across Life History Stages of Hippodemia convergens

76

Hippodemia convergens, also called the convergent lady beetle is used extensively in biological control of aphids, whiteflies, and scale insects. Unlike most lady beetles, the convergent beetle displays aggregation behaviors during the winter. Beetles are commonly collected in this area and distributed for biological control. We hypothesize that transcriptome data could lead to a better understanding of the genes involved in their convergent behaviors. We preformed whole RNA extractions from flash frozen homogenized tissue of instars, pupae, and adults of an Eastern (Kansas City, MO) population of Hippodamia convergens for further analysis of gene expression profiling across life history stages. Whole RNA was then subjected to NGS library preparation. Only two of the samples yielded quality libraries for sequencing. Our future research plans are to send the prepared libraries for sequencing and compile and annotate the transcriptome of H. convergens. Transcriptomic data could potentially be used to isolate compounds associated with H. convergens aggregation behavior and be of use within the biological control industry to attract lady beetles as natural enemies.