Presentation Title

Gene flow dynamics and phylogenetics of Zostera marina atam

Faculty Mentor

Frank Emanuele

Start Date

23-11-2019 8:45 AM

End Date

23-11-2019 9:30 AM

Location

78

Session

poster 2

Type of Presentation

Poster

Subject Area

biological_agricultural_sciences

Abstract

Gene transcription changes occur over time, more in areas where Zostera species are not adapted to higher temperatures. Z. marina atam is located in Mar de Cortez of Bahia de Kino, Sonora, Mexico. Prescott College and the Comcaac tribe have created theoretical maps using Comcaac tribal knowledge and satellite data to create predictions of where Z. marina atam will be found within the Canal de Infiernillo. Muniz-Salazar, et. al. (2005) mentioned that gene flow and genetic variation is high around the western coast of Baja California but even higher within the eastern coast in the Mar de Cortez. If the genetics of Z. marina atam can be evaluated and its genetic properties can be determined, then it can be compared to Z. marina from around the Pacific. Having obtained samples from 6 different locations, 4 of which were located in dense areas and 2 from sparse areas, DNA was extracted to evaluate the genetics of Z. marina atam in order to determine its genetic properties and compare these to Z. marina from around the pacific using the minION sequencing technology to sequence as much of the genome available via this technology. Also using flow cell technology provides readings of nucleotide sequences, and these data provide us with the genome information. A sequencing protocol called rapid sequencing was used first but when the DNA fragments were too small, another sequencing protocol, ligation, was utilized to generate longer sequences to correct the problems of the previous protocol. Since the ligation protocol is still ongoing, the sequences have not been analyzed. In the future when long segments of DNA are sequenced, Polymerase Chain Reaction (PCR) will be used to copy specific genes to further compare the genetic properties of Z. marina atam and the Z. marina of the Pacific.

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Nov 23rd, 8:45 AM Nov 23rd, 9:30 AM

Gene flow dynamics and phylogenetics of Zostera marina atam

78

Gene transcription changes occur over time, more in areas where Zostera species are not adapted to higher temperatures. Z. marina atam is located in Mar de Cortez of Bahia de Kino, Sonora, Mexico. Prescott College and the Comcaac tribe have created theoretical maps using Comcaac tribal knowledge and satellite data to create predictions of where Z. marina atam will be found within the Canal de Infiernillo. Muniz-Salazar, et. al. (2005) mentioned that gene flow and genetic variation is high around the western coast of Baja California but even higher within the eastern coast in the Mar de Cortez. If the genetics of Z. marina atam can be evaluated and its genetic properties can be determined, then it can be compared to Z. marina from around the Pacific. Having obtained samples from 6 different locations, 4 of which were located in dense areas and 2 from sparse areas, DNA was extracted to evaluate the genetics of Z. marina atam in order to determine its genetic properties and compare these to Z. marina from around the pacific using the minION sequencing technology to sequence as much of the genome available via this technology. Also using flow cell technology provides readings of nucleotide sequences, and these data provide us with the genome information. A sequencing protocol called rapid sequencing was used first but when the DNA fragments were too small, another sequencing protocol, ligation, was utilized to generate longer sequences to correct the problems of the previous protocol. Since the ligation protocol is still ongoing, the sequences have not been analyzed. In the future when long segments of DNA are sequenced, Polymerase Chain Reaction (PCR) will be used to copy specific genes to further compare the genetic properties of Z. marina atam and the Z. marina of the Pacific.