Presentation Title

Development of an Alternative Food Source for Farm-Raised Fish by Selective Breeding of the Unicellular Green Alga Chlamydomonas

Faculty Mentor

Amybeth Cohen

Start Date

23-11-2019 8:45 AM

End Date

23-11-2019 9:30 AM

Location

82

Session

poster 2

Type of Presentation

Poster

Subject Area

biological_agricultural_sciences

Abstract

To lessen overfishing and increase the production of high-quality fish for human consumption, we have moved as a global society toward improving aquaculture (fish farming). Aquaculture is a method to produce high-quality farm-raised fish in a controlled and regulated environment. In 2016, aquaculture contributed to 43% of total fish production worldwide, which is approximately 232 billion dollars (FAO, The State of World Fisheries and Aquaculture, 2018). To supplement the growth of farm-raised fish, aquaculture industries use fishmeal (FM), which is typically composed of smaller, ground-up marine fish, and has a high production cost (FAO, Fishmeal, 2019). The use of ocean resources for FM is unsustainable and damages the marine ecosystem. Therefore, the goal of this study is to develop an alternative food source for farm-raised fish using selective breeding methods of Chlamydomonas spp. to produce a strain with high omega-3, lipid, and protein content. Forty-five strains of Chlamydomonas were received from collection centers across the world. Gametogenesis, which is seen as the transition from stationary to motile cells, was induced by growing the strains on nitrogen deficient Tris-Acetate-Phosphate (TAP) medium. For each strain, the optimal time required to reach the highest percent motility after the induction of gametogenesis was determined, followed by mating strains of opposite mating types. While mating of laboratory strains was successful, the mating of non-laboratory strains from diverse, geographical locations was not successful. Thus, we are exploring other stress agents, such as high light, low temperature and a no-carbon containing media to induce gametogenesis. For future steps, Chlamydomonas cells will be treated with gamete-activating drugs, such as dibutyryl-cAMP and papaverine, to promote mating of non-laboratory strains. Once successful mating is achieved, the progeny can be screened for omega-3, lipid, and protein content.

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Nov 23rd, 8:45 AM Nov 23rd, 9:30 AM

Development of an Alternative Food Source for Farm-Raised Fish by Selective Breeding of the Unicellular Green Alga Chlamydomonas

82

To lessen overfishing and increase the production of high-quality fish for human consumption, we have moved as a global society toward improving aquaculture (fish farming). Aquaculture is a method to produce high-quality farm-raised fish in a controlled and regulated environment. In 2016, aquaculture contributed to 43% of total fish production worldwide, which is approximately 232 billion dollars (FAO, The State of World Fisheries and Aquaculture, 2018). To supplement the growth of farm-raised fish, aquaculture industries use fishmeal (FM), which is typically composed of smaller, ground-up marine fish, and has a high production cost (FAO, Fishmeal, 2019). The use of ocean resources for FM is unsustainable and damages the marine ecosystem. Therefore, the goal of this study is to develop an alternative food source for farm-raised fish using selective breeding methods of Chlamydomonas spp. to produce a strain with high omega-3, lipid, and protein content. Forty-five strains of Chlamydomonas were received from collection centers across the world. Gametogenesis, which is seen as the transition from stationary to motile cells, was induced by growing the strains on nitrogen deficient Tris-Acetate-Phosphate (TAP) medium. For each strain, the optimal time required to reach the highest percent motility after the induction of gametogenesis was determined, followed by mating strains of opposite mating types. While mating of laboratory strains was successful, the mating of non-laboratory strains from diverse, geographical locations was not successful. Thus, we are exploring other stress agents, such as high light, low temperature and a no-carbon containing media to induce gametogenesis. For future steps, Chlamydomonas cells will be treated with gamete-activating drugs, such as dibutyryl-cAMP and papaverine, to promote mating of non-laboratory strains. Once successful mating is achieved, the progeny can be screened for omega-3, lipid, and protein content.