Presentation Title

The determination of significant factors that contribute to the chemical cell disruption of the microalgal species, Nannochloropsis oculata

Faculty Mentor

Dominique Ingato

Start Date

23-11-2019 8:45 AM

End Date

23-11-2019 9:30 AM

Location

122

Session

poster 2

Type of Presentation

Poster

Subject Area

biological_agricultural_sciences

Abstract

Biofuel commercialization is complicated because of the cell disruption step within its oil extraction process. The purpose of this study was to identify factors that affect chemical cell disruption of the microalgal species, Nannochloropsis oculata. The factors tested for significance were pretreatment method, buffer type, buffer volume, incubation time, and incubation temperature. Chlorophyll a is the only type of chlorophyll present in this species and has an absorption band at 662nm. Therefore, a spectrophotometer was used to measure the optical density of the lysate supernatant as the response variable. JMP Statistical Software was used to analyze the results via a Resolution III screening design: a half factorial design with four center points. A resolution III half fractional factorial design was chosen to examine the relationship of each factor on the response variable using the minimal number of independent experiments. Center points were added to assess the need to include curvature in the model. Buffer type and volume were found to be the two most significant factors. Using 5 ml of ethanol to lyse 5 ml of algae sample volume proved the most successful in lysing cells. The oil extraction process of biofuel manufacturing is expensive and time-consuming because of the sheer volume of the cells that are needed in order to commercialize biofuel. Therefore, the optimization of the cell disruption method would improve biofuel production economically by increasing its efficiency. Additional buffers will be explored in future experiments.

Keywords: chemical cell disruption; chlorophyll a; Nannochloropsis oculata; half fractional factorial; screening design

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Nov 23rd, 8:45 AM Nov 23rd, 9:30 AM

The determination of significant factors that contribute to the chemical cell disruption of the microalgal species, Nannochloropsis oculata

122

Biofuel commercialization is complicated because of the cell disruption step within its oil extraction process. The purpose of this study was to identify factors that affect chemical cell disruption of the microalgal species, Nannochloropsis oculata. The factors tested for significance were pretreatment method, buffer type, buffer volume, incubation time, and incubation temperature. Chlorophyll a is the only type of chlorophyll present in this species and has an absorption band at 662nm. Therefore, a spectrophotometer was used to measure the optical density of the lysate supernatant as the response variable. JMP Statistical Software was used to analyze the results via a Resolution III screening design: a half factorial design with four center points. A resolution III half fractional factorial design was chosen to examine the relationship of each factor on the response variable using the minimal number of independent experiments. Center points were added to assess the need to include curvature in the model. Buffer type and volume were found to be the two most significant factors. Using 5 ml of ethanol to lyse 5 ml of algae sample volume proved the most successful in lysing cells. The oil extraction process of biofuel manufacturing is expensive and time-consuming because of the sheer volume of the cells that are needed in order to commercialize biofuel. Therefore, the optimization of the cell disruption method would improve biofuel production economically by increasing its efficiency. Additional buffers will be explored in future experiments.

Keywords: chemical cell disruption; chlorophyll a; Nannochloropsis oculata; half fractional factorial; screening design