Presentation Title

Pacemaker Assisted Cardiogenesis on CAD Printed Substrate to Produce a Beating “Cardiothread” Therapeutic Suture

Faculty Mentor

Dr. James Harber

Start Date

23-11-2019 10:00 AM

End Date

23-11-2019 10:45 AM

Location

49

Session

poster 3

Type of Presentation

Poster

Subject Area

biological_agricultural_sciences

Abstract

This project aims to expand on how stem cells can be used to create a beating patch of cardiac tissue assisted by a lab built pacemaker. The surgical replacement of dead myocardium and enhancement of heart regeneration post-surgery with stem cell therapies is now clinically practical. Computer-assisted designed (CAD) 3D printed disk-shaped structures were created to hold the sutures in place and support the growth of cells on the sutures. The CAD device fits into a six-well tissue culture dish that delivers the pacemaker charge to the cells via platinum electrodes. The experimental cell culture of P19.CL6 mouse pluripotent cells differentiate into cardiac cells within 14 days in the presence of 1% DMSO. The hypothesis of this experiment is that differentiated cells would beat in an ultra-low adherent six-well petri dish that is covered by a 3D printed lid connected to the pacemaker. The lid was designed to direct an electric current through the disks that house the cells in order to stimulate a more uniform beating of the cardiomyocytes. CAD and Fusion 360 software programs were utilized to create the CAD design, printing was done on a Lulzbot TAZ 6 3D printer using PLA filament. The results showed significant growth of cardiac cells on the sutures and between the tissue blocks in the rectangular matrix along the five parallel sutures. However, more experimental work needs to be done to encourage the parallel growth of beating heart tissue using the pacemaker.

This document is currently not available here.

Share

COinS
 
Nov 23rd, 10:00 AM Nov 23rd, 10:45 AM

Pacemaker Assisted Cardiogenesis on CAD Printed Substrate to Produce a Beating “Cardiothread” Therapeutic Suture

49

This project aims to expand on how stem cells can be used to create a beating patch of cardiac tissue assisted by a lab built pacemaker. The surgical replacement of dead myocardium and enhancement of heart regeneration post-surgery with stem cell therapies is now clinically practical. Computer-assisted designed (CAD) 3D printed disk-shaped structures were created to hold the sutures in place and support the growth of cells on the sutures. The CAD device fits into a six-well tissue culture dish that delivers the pacemaker charge to the cells via platinum electrodes. The experimental cell culture of P19.CL6 mouse pluripotent cells differentiate into cardiac cells within 14 days in the presence of 1% DMSO. The hypothesis of this experiment is that differentiated cells would beat in an ultra-low adherent six-well petri dish that is covered by a 3D printed lid connected to the pacemaker. The lid was designed to direct an electric current through the disks that house the cells in order to stimulate a more uniform beating of the cardiomyocytes. CAD and Fusion 360 software programs were utilized to create the CAD design, printing was done on a Lulzbot TAZ 6 3D printer using PLA filament. The results showed significant growth of cardiac cells on the sutures and between the tissue blocks in the rectangular matrix along the five parallel sutures. However, more experimental work needs to be done to encourage the parallel growth of beating heart tissue using the pacemaker.