Presentation Title

Herbicide induced reproductive dysfunction in fruit fly Drosophila melanogaster

Presenter Information

Jacob SmithFollow

Faculty Mentor

Anathbandhu Chaudhuri

Start Date

23-11-2019 10:00 AM

End Date

23-11-2019 10:45 AM

Location

59

Session

poster 3

Type of Presentation

Poster

Subject Area

biological_agricultural_sciences

Abstract

Spectracide is a very commonly used herbicide that contains four harmful components viz., atrazine, diquat dibromide, fluazifop-p-butyle and dicamba that are banned in various European countries but still permitted in the United States. These active ingredients in spectracide are suspected to cause neurological disorders and may have an adverse effect on reproductive function. Our previous experiments report that spectracide disrupts the biological clock in the fly model and creates oxidative stress. This experiment is designed to find out the effects of spectracide on reproductive behavior and performances using the fruit fly, Drosophila melanogaster.

Young adult male and female flies (5-7 days old) were fed with 10% spectracide solution mixed with 5% sucrose for six hours then the grooming behavior was studied at 6, 12 and 24 hours. The control flies were fed with only 5% sucrose solution. We observe that male grooming behavior in the presence of females is severely affected by spectracide (1 time/ minute) when compared to controls (4 times / minute). Also, spectracide fed flies produce a very little amount of progeny with a significant delay in the larval development compared to control. Since grooming activity is a major event by males prior to mating, we hypothesize that the low numbers of progeny could be due to either disruption in fertility or defects in reproductive development caused by the active ingredients of the spectracide when exposed for six hours. Histopathology of adult reproductive system and the sperm motility will help in better understanding the actual deleterious effect of spectracide which are under progress. Taken together, these results suggest that elements of spectracide have a negative effect on reproductive behavior and outcome in Drosophila melanogaster which could also be harmful to humans.

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Nov 23rd, 10:00 AM Nov 23rd, 10:45 AM

Herbicide induced reproductive dysfunction in fruit fly Drosophila melanogaster

59

Spectracide is a very commonly used herbicide that contains four harmful components viz., atrazine, diquat dibromide, fluazifop-p-butyle and dicamba that are banned in various European countries but still permitted in the United States. These active ingredients in spectracide are suspected to cause neurological disorders and may have an adverse effect on reproductive function. Our previous experiments report that spectracide disrupts the biological clock in the fly model and creates oxidative stress. This experiment is designed to find out the effects of spectracide on reproductive behavior and performances using the fruit fly, Drosophila melanogaster.

Young adult male and female flies (5-7 days old) were fed with 10% spectracide solution mixed with 5% sucrose for six hours then the grooming behavior was studied at 6, 12 and 24 hours. The control flies were fed with only 5% sucrose solution. We observe that male grooming behavior in the presence of females is severely affected by spectracide (1 time/ minute) when compared to controls (4 times / minute). Also, spectracide fed flies produce a very little amount of progeny with a significant delay in the larval development compared to control. Since grooming activity is a major event by males prior to mating, we hypothesize that the low numbers of progeny could be due to either disruption in fertility or defects in reproductive development caused by the active ingredients of the spectracide when exposed for six hours. Histopathology of adult reproductive system and the sperm motility will help in better understanding the actual deleterious effect of spectracide which are under progress. Taken together, these results suggest that elements of spectracide have a negative effect on reproductive behavior and outcome in Drosophila melanogaster which could also be harmful to humans.