Presentation Title

Induced Pluripotent Stem Cells Express Major Histocompatibility Complex Class I Genes in its Pluripotent Stage

Faculty Mentor

Bianca Mothe

Start Date

23-11-2019 10:00 AM

End Date

23-11-2019 10:45 AM

Location

67

Session

poster 3

Type of Presentation

Poster

Subject Area

biological_agricultural_sciences

Abstract

Induced pluripotent stem cells (IPSCs) have been gaining popularity in the past decade. The ability to take any type of adult cell on the human body and to reprogram it back to its pluripotent state has created a plethora of opportunities in the field of regenerative medicine. These pluripotent stem cells can then be differentiated into almost any cell type desired, which can ultimately be used clinically for cell-based therapy. Although this method is promising, it requires a lot of time, effort, and money. Research has recently moved towards the idea of a universal stem cell line that can be mass-produced and readily available to any patient in need. To do this, the universal stem cell lines would need to have a lack of Major Histocompatibility Complex (MHC) Class I genes, otherwise, the human immune system would reject these cells. There is no current evidence of a timeline of MHC expression in IPSCs. Previous studies show low levels of MHC expression on ES and IPS cells, therefore similarly we hypothesized that at the pluripotent stem cell stage, there would be little or no expression of MHC on the surface of the cells. We tested a commonly used, commercially available, IPSC line for MHC expression using flow cytometry for the presence of MHC. We now have evidence that there is MHC expression in an IPSC line, even in the pluripotency stage. We are currently testing these IPSCs to better understand the nature of MHC class I genes and to help guide research in the production of universal stem cell lines.

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Nov 23rd, 10:00 AM Nov 23rd, 10:45 AM

Induced Pluripotent Stem Cells Express Major Histocompatibility Complex Class I Genes in its Pluripotent Stage

67

Induced pluripotent stem cells (IPSCs) have been gaining popularity in the past decade. The ability to take any type of adult cell on the human body and to reprogram it back to its pluripotent state has created a plethora of opportunities in the field of regenerative medicine. These pluripotent stem cells can then be differentiated into almost any cell type desired, which can ultimately be used clinically for cell-based therapy. Although this method is promising, it requires a lot of time, effort, and money. Research has recently moved towards the idea of a universal stem cell line that can be mass-produced and readily available to any patient in need. To do this, the universal stem cell lines would need to have a lack of Major Histocompatibility Complex (MHC) Class I genes, otherwise, the human immune system would reject these cells. There is no current evidence of a timeline of MHC expression in IPSCs. Previous studies show low levels of MHC expression on ES and IPS cells, therefore similarly we hypothesized that at the pluripotent stem cell stage, there would be little or no expression of MHC on the surface of the cells. We tested a commonly used, commercially available, IPSC line for MHC expression using flow cytometry for the presence of MHC. We now have evidence that there is MHC expression in an IPSC line, even in the pluripotency stage. We are currently testing these IPSCs to better understand the nature of MHC class I genes and to help guide research in the production of universal stem cell lines.