Presentation Title

Ancestral or inverted? A PCR-based test effectively contrasts alternative mitochondrial gene orders in chitons

Faculty Mentor

Dr. Douglas J. Eernisse

Start Date

23-11-2019 10:00 AM

End Date

23-11-2019 10:45 AM

Location

91

Session

poster 3

Type of Presentation

Poster

Subject Area

biological_agricultural_sciences

Abstract

Author: Kaitlyn Manh, California State University Fullerton Mentor: Douglas J. Eernisse, PhD., Department of Biological Science

Chitons are marine mollusks that have eight shell plates, and typically graze on algae in rocky marine habitats. The phylogeny of chiton species within the family, Lepidochitonidae, is still unresolved, and competing classifications are in use. Previous sequencing of complete mitochondrial genomes revealed that two genera within this family share an inverted cluster of tRNA genes, relative to the ancestral gene order of this tRNA cluster found in other chitons and even other molluscan classes. We used PCR with custom pairs of primers to test other species within the family and select species from other families, in order to see which have either the ancestral or inverted versions of this tRNA cluster gene order. Species with this derived inversion were inferred to have shared a common ancestor already with this inversion. Our results agreed with separate phylogenetic estimates from mitochondrial gene sequence comparisons. We have confirmed that only some but not all Lepidochitonidae genera belong to a monophyletic subgroup of the family with the derived inverted mitochondrial gene order. We are continuing to test selected species or genera to better resolve Lepidochitonidae phylogeny and to gain a better understanding of their current geographical distribution and success through time.

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Nov 23rd, 10:00 AM Nov 23rd, 10:45 AM

Ancestral or inverted? A PCR-based test effectively contrasts alternative mitochondrial gene orders in chitons

91

Author: Kaitlyn Manh, California State University Fullerton Mentor: Douglas J. Eernisse, PhD., Department of Biological Science

Chitons are marine mollusks that have eight shell plates, and typically graze on algae in rocky marine habitats. The phylogeny of chiton species within the family, Lepidochitonidae, is still unresolved, and competing classifications are in use. Previous sequencing of complete mitochondrial genomes revealed that two genera within this family share an inverted cluster of tRNA genes, relative to the ancestral gene order of this tRNA cluster found in other chitons and even other molluscan classes. We used PCR with custom pairs of primers to test other species within the family and select species from other families, in order to see which have either the ancestral or inverted versions of this tRNA cluster gene order. Species with this derived inversion were inferred to have shared a common ancestor already with this inversion. Our results agreed with separate phylogenetic estimates from mitochondrial gene sequence comparisons. We have confirmed that only some but not all Lepidochitonidae genera belong to a monophyletic subgroup of the family with the derived inverted mitochondrial gene order. We are continuing to test selected species or genera to better resolve Lepidochitonidae phylogeny and to gain a better understanding of their current geographical distribution and success through time.