Presentation Title

Developing a Defined Diet for White-Lined Sphinx Moth Nutritional Studies

Faculty Mentor

Dustin VanOverbeke

Start Date

23-11-2019 10:00 AM

End Date

23-11-2019 10:45 AM

Location

97

Session

poster 3

Type of Presentation

Poster

Subject Area

biological_agricultural_sciences

Abstract

The purpose of this experiment was to create a defined diet for the white lined sphinx moth, Hyles lineata (Fabricius, 1775), for later use in nutritional choice studies. Newly molted 5th instar caterpillars were reared on two defined diets, one free of formaldehyde and the other with formaldehyde. Diet changes and weighing of caterpillars, leftover food, and frass were performed every three days. We found that caterpillars ate significantly more (p<0.0001) and grew significantly bigger (p<0.001) on the diet lacking formaldehyde. However, 100% of caterpillars on non-formaldehyde diet had severe developmental problems, likely due to infection. Formaldehyde is used in many insect diets but it appeared to be an inhibitor to feeding in H. lineata diets. However, the use of an effective antimicrobial agent is necessary, because of the potential for disease in the colony.

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Nov 23rd, 10:00 AM Nov 23rd, 10:45 AM

Developing a Defined Diet for White-Lined Sphinx Moth Nutritional Studies

97

The purpose of this experiment was to create a defined diet for the white lined sphinx moth, Hyles lineata (Fabricius, 1775), for later use in nutritional choice studies. Newly molted 5th instar caterpillars were reared on two defined diets, one free of formaldehyde and the other with formaldehyde. Diet changes and weighing of caterpillars, leftover food, and frass were performed every three days. We found that caterpillars ate significantly more (p<0.0001) and grew significantly bigger (p<0.001) on the diet lacking formaldehyde. However, 100% of caterpillars on non-formaldehyde diet had severe developmental problems, likely due to infection. Formaldehyde is used in many insect diets but it appeared to be an inhibitor to feeding in H. lineata diets. However, the use of an effective antimicrobial agent is necessary, because of the potential for disease in the colony.