Presentation Title

piRNA Required for Stress Granules in late Pachytene Stage.

Presenter Information

Isaias TorresFollow

Faculty Mentor

Heejin Choi, Jurrien Dean

Start Date

23-11-2019 10:00 AM

End Date

23-11-2019 10:45 AM

Location

179

Session

poster 3

Type of Presentation

Poster

Subject Area

health_nutrition_clinical_science

Abstract

Spermatogenesis is a highly specialized process that carries correct epigenetic and genetic information to the next generation. Male mice models have reported the presence of small non-coding RNAs (sncRNA) that facilitate in spermatogenesis. One of the most prominent sncRNAs precisely depicted is the germline-enriched piwi-interacting RNA (piRNAs). Piwi proteins (MILI, MIWI, MIWI2) that bind to piRNAs are distinctly established in the germline. It has been reported that piRNAs are important regulators of the male germ cell differentiation and degradation of transposons. An essential role of the cellular response to environmental stress is the abundance of stress granules (SGs) that take place in the cytoplasm. Acute heat stress and oxidative stress induces the formation of SGs. Further interpretation of piRNAs role will eventually help facilitate the underlying affects of human male infertility. We sought to investigate whether the elimination of piRNA creates an immune response such as SG formation and if so, what stage specificity in spermatogenesis does this occur? To investigate, we used crossbreed piRNA eliminated mutant mice generated by CRISP/Cas9 and genotype PCR to confirm piRNA KO in male mice. An Immunohistochemical (IHC) analysis was conducted using anti-TIA-1 as the SG marker and a Western Blot analysis to evaluate protein activity in male mice testes. Our analysis revealed an abundance of SGs in the late pachytene stage in spermatogenesis and lower protein level activation in KO mice. Our results suggest that KO of piRNA leads to male infertility and an immune response.

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Nov 23rd, 10:00 AM Nov 23rd, 10:45 AM

piRNA Required for Stress Granules in late Pachytene Stage.

179

Spermatogenesis is a highly specialized process that carries correct epigenetic and genetic information to the next generation. Male mice models have reported the presence of small non-coding RNAs (sncRNA) that facilitate in spermatogenesis. One of the most prominent sncRNAs precisely depicted is the germline-enriched piwi-interacting RNA (piRNAs). Piwi proteins (MILI, MIWI, MIWI2) that bind to piRNAs are distinctly established in the germline. It has been reported that piRNAs are important regulators of the male germ cell differentiation and degradation of transposons. An essential role of the cellular response to environmental stress is the abundance of stress granules (SGs) that take place in the cytoplasm. Acute heat stress and oxidative stress induces the formation of SGs. Further interpretation of piRNAs role will eventually help facilitate the underlying affects of human male infertility. We sought to investigate whether the elimination of piRNA creates an immune response such as SG formation and if so, what stage specificity in spermatogenesis does this occur? To investigate, we used crossbreed piRNA eliminated mutant mice generated by CRISP/Cas9 and genotype PCR to confirm piRNA KO in male mice. An Immunohistochemical (IHC) analysis was conducted using anti-TIA-1 as the SG marker and a Western Blot analysis to evaluate protein activity in male mice testes. Our analysis revealed an abundance of SGs in the late pachytene stage in spermatogenesis and lower protein level activation in KO mice. Our results suggest that KO of piRNA leads to male infertility and an immune response.