Presentation Title

Genetic identification of Malayasian Amusium pleuronectes through the use of phylogenetics

Faculty Mentor

Alvin Alejandrino

Start Date

23-11-2019 10:45 AM

End Date

23-11-2019 11:30 AM

Location

64

Session

poster 4

Type of Presentation

Poster

Subject Area

biological_agricultural_sciences

Abstract

Amusium pleuronectes, also known as Asian moon scallops, are found throughout the Indo-Pacific Ocean. Due to Asian moon scallops abundance off the shores of Indonesia, Thailand, Malaysia and other countries, the scallops are widely harvested and consumed. Asian moon scallops are identified by their moderate size, disc-shaped shell, and the internal ribbing on both valves. However, these characters alone are inadequate in identifying species of scallops due to convergent evolution. Unrelated species, Ylistrum balloti and Y. japonicum, are also found in the Indo-Pacific Ocean. Y. balloti and Y. japonicum are also harvested and consumed, and exhibit similar morphology as the Asian moon scallop. For this reason, there is a need to examine the genetic material of these commercially-important scallops to properly identify them and ensure a sustainable fishery for the future. Additionally, there are certain geographical barriers that need to be taken into consideration. The geography of the Indo-Pacific Ocean is complex and has been shown to separate populations of Asian moon scallops. In this study, the genetic material of 30 individual scallops from Malaysia were studied to determine whether they are true Asian moon scallops and whether they consist of multiple non-interbreeding populations. We hypothesize that scallops collected from Malaysia are Asian moon scallops and do interbreed with some populations in the Indo-Pacific Ocean. The adductor muscles of the specimens were excised for the isolation of DNA. The nuclear (histone H3 and 28S ribosomal DNA) and mitochondrial (16S ribosomal DNA, 12S ribosomal DNA, and cytochrome oxidase I) genes were amplified using polymerase chain reaction. Genbank was used to obtain sequences of other scallops and outgroups. Maximum parsimony, maximum likelihood, and Bayesian inference analyses were utilized to reconstruct phylogenetic trees. Preliminary results support our hypothesis that the sample of scallops from Malaysia are indeed Amusium pleuronectes and also true scallops. There is evidence that they interbreed with other Asian moon scallops in the Indo-Pacific Ocean, but additional analyses need to be conducted to confirm this.

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Nov 23rd, 10:45 AM Nov 23rd, 11:30 AM

Genetic identification of Malayasian Amusium pleuronectes through the use of phylogenetics

64

Amusium pleuronectes, also known as Asian moon scallops, are found throughout the Indo-Pacific Ocean. Due to Asian moon scallops abundance off the shores of Indonesia, Thailand, Malaysia and other countries, the scallops are widely harvested and consumed. Asian moon scallops are identified by their moderate size, disc-shaped shell, and the internal ribbing on both valves. However, these characters alone are inadequate in identifying species of scallops due to convergent evolution. Unrelated species, Ylistrum balloti and Y. japonicum, are also found in the Indo-Pacific Ocean. Y. balloti and Y. japonicum are also harvested and consumed, and exhibit similar morphology as the Asian moon scallop. For this reason, there is a need to examine the genetic material of these commercially-important scallops to properly identify them and ensure a sustainable fishery for the future. Additionally, there are certain geographical barriers that need to be taken into consideration. The geography of the Indo-Pacific Ocean is complex and has been shown to separate populations of Asian moon scallops. In this study, the genetic material of 30 individual scallops from Malaysia were studied to determine whether they are true Asian moon scallops and whether they consist of multiple non-interbreeding populations. We hypothesize that scallops collected from Malaysia are Asian moon scallops and do interbreed with some populations in the Indo-Pacific Ocean. The adductor muscles of the specimens were excised for the isolation of DNA. The nuclear (histone H3 and 28S ribosomal DNA) and mitochondrial (16S ribosomal DNA, 12S ribosomal DNA, and cytochrome oxidase I) genes were amplified using polymerase chain reaction. Genbank was used to obtain sequences of other scallops and outgroups. Maximum parsimony, maximum likelihood, and Bayesian inference analyses were utilized to reconstruct phylogenetic trees. Preliminary results support our hypothesis that the sample of scallops from Malaysia are indeed Amusium pleuronectes and also true scallops. There is evidence that they interbreed with other Asian moon scallops in the Indo-Pacific Ocean, but additional analyses need to be conducted to confirm this.